Dr. Rajpurohit Lab is working on the Experimental Therapeutic in Glioblastoma and
Cardio-oncology by using in-vivo transparent transgenic zebrafish and mouse model which includes high throughput (HT)
drug discovery, repurposing of FDA approved drugs, identification of novel hit and
lead drugs, drug validation and drug toxicity in glioblastoma and cardio oncology
therapeutics.
Dr. Rajpurohit Lab developing and establishing in-vivo transparent transgenic cellular
phenotype model of zebrafish to study of the cell death process (Annexin5 and Inflammatory
activity NFkB in the microglia-resident macrophage of the brain and cardiac myocytes.
In cardio-oncology, our research is on the development of a transparent transgenic
zebrafish model to study Annexin-5 and NF-kB activity in normal and inflamed cardiac
myocytes. In glioblastoma research, his lab determines the role of NFkB and microglia
function under normal healthy status and in glioblastomas by using mouse and zebrafish
model. He is establishing the role of annexin-5 and NF-kB activity in the cardiovascular function under normal and in metabolic aberration
by developing homozygous casper/ myl7:RFP; annexin-5:YFP/NF-kB:GFP transgenic zebrafish.
Dr. Rajpurohit designed and established the novel whole organism based ARQiv (automated
reporter based quantitative in-vivo HT-screen) platform. This novel devise facilitates
large-scale HT drug or chemical screen to find the hit and lead drug for targeted
disease module. He has successfully applied this unique platform to screen over 3200
drug candidates to identify novel pathway for increasing pancreatic beta-cell neogenesis
and proliferation.
Investigator Initiated Grant 2022 (July 2022-July 2025)
Foundation Grant: Kirk Kimmerling Foundation 2022 (July 2022-June 2025)
Title of the Project Validation and Toxicology Study of K21 Drug in Zebrafish Model:
The grant has been awarded for the project “Validation and Toxicology Study of K21
Drug in Zebrafish Model”.
Augusta University Research and Training Grants Awarded
2022 CURS Spring Material Grant. Study Title: Generation of Casper/Annexin5 (F05 Homozygous Progeny) Transparent Transgenic
Zebrafish Strain to Study Cardiomyocyte Activity
2021 CURS Fall Material Grant. Study Title: Generation of Casper/Annexin5 (F05 Homozygous Progeny) Transparent Transgenic
Zebrafish Strain to Study Cardiomyocyte Activity
2021 CURS Spring Material Grant. Study Title: Generation of Casper/Annexin5 (F05 Homozygous Progeny) Transparent Transgenic
Zebrafish Strain to Study Cardiomyocyte Activity
2020 CURS Fall Material Grant. Study Title: In-vivo Confocal imaging of newly Developed Homozygous Casper (Transparent
skin)-Transgenic Zebrafish Progeny to Study Microglia Function
2020 CURS Summer Scholars Program Grant. Project Title: Generation of Transparent Transgenic Zebrafish Homozygous Progeny
to study the Microglia Function in Health and Disease Status
2019 CURS Summer Scholars Program Grant. Project Title: Development of Transparent Transgenic Zebrafish Model to Investigate
of Microglia Function.
Doughty D, Rajpurohit SK Trang A, Alptekin A, Korkaya A, Achyut B, Arbab A, and Bradford
J. Development of a Novel Purification Protocol to Isolate and Identify Brain Microglia.
Experimental Biology and Medicine. 2022. PMID: 35666093. DOI: 10.1177/15353702221096060.
Rajpurohit SK, Gopal A, Mon MY, Patel NG, Arora V. Development of Tg(UAS:SEC-Hsa.ANXA5-YFP,myl7:RFP); Casper(roy−/−,nacre−/−)Transparent Transgenic In Vivo Zebrafish Model to Study the Cardiomyocyte Function. Cells. 2021; 10(8):1963. https://doi.org/10.3390/cells10081963
Ullah A, Doroodchi A, Velasquez Zarate L, Mattox SN, Sliker T, Willhite DK, Khan J,
Owen HC, Rajpurohit SK, Patel NG, Hatley RM. Metastatic Neuroblastoma Presenting as
a Submandibular Mass with Mandibular Bone Involvement in a Three-Year-Old Child. International Journal of Environmental Research and Public Health. 2021; 18(8):4157. https://doi.org/10.3390/ijerph18084157
D White, A Eroglu , G Wang , L Zhang , S Sengupta , SK Rajpurohit , S Walker, J Qian
and JS Mumm. A Versatile Whole-organism Screening Platform enabling in vivo Drug Discovery
at High-throughput Rates: ARQiv-HTS. Nature Protocols. 2016; Vol.11 No12: 2432-2453.
G Wanga, SK Rajpurohit, F Delasprea, SL. Walker, DT White, A Ceasrine, R Kuruvilla,
Ruo-jing Lid, JS. Shime, JO. Liud, MJ. Parsons, and JS. Mumm. First quantitative high-throughput
screen in zebrafish identifies novel pathways for increasing pancreatic β-cell mass.
Elife. 2015 Jul 28;4. doi: 10.7554/eLife.08261
Urban BE, Neogi P, Senthilkumar K, Rajpurohit SK, Jagadeeswaran P, Kim S, Fujita Y,
Neogi A. Bioimaging using the optimized nonlinear optical properties of zno nanoparticles.
Selected Topics in Quantum Electronics, IEEE Journal of. 2012;18:1451-1456
Jagadeeswaran P, Carrillo M, Radhakrishnan UP, Rajpurohit SK, Kim S. Laser-induced
thrombosis in zebrafish. Methods in cell biology. 2011;101:197
Carrillo M, Kim S, Rajpurohit SK, Kulkarni V, Jagadeeswaran P. Zebrafish von Willebrand
factor. Blood Cells, Molecules, and Diseases. 2010;45:326-333
Kim S, Radhakrishnan UP, Rajpurohit SK, Kulkarni V, Jagadeeswaran P. Vivo-morpholino
knockdown of αiib: A novel approach to inhibit thrombocyte function in adult zebrafish.
Blood Cells, Molecules, and Diseases. 2010;44:169-174
Kim S, Carlson R, Zafreen L, Rajpurohit SK, Jagadeeswaran P. Modular, easy-to-assemble,
low-cost zebrafish facility. Zebrafish. 2009;6:269-274
Singh P, Pendse A, Ahmed A, Ramavataram D, Rajpurohit SK. A study of recurrent stone
formers with special reference to renal tubular acidosis. Urological Research. 1995;23:201-203
Rajpurohit SK, Pendse A, Ahmed A, Singh P. Complete distal renal tubular acidosis:
Biochemical profile of a case. Indian Journal of Clinical Biochemistry. 1992;7:205-206.
Ahmed A, Pendse A
Rajpurohit SK, Singh P. Assessment of renal acidification capacity of stone formers
by acid challenge test. Indian Journal of Clinical Biochemistry. 1992;7:171-178.
