The Miano lab has developed dozens of rodent models, primarily with gene editing platforms, to study previously challenging research questions in vascular smooth muscle cell pathobiology. Current topics of interest include the control of smooth muscle gene transcription in vivo, vascular smooth muscle cell-restricted gene knockouts, Myocardin protein biology, and the ACE2/ACE2-DT gene pair in SARS-CoV-2 infection and (long) COVID-19 disease.
Transcriptional/post-transcriptional regulation of the SRF-Myocardin switch
Function and regulation of novel SRF-Myocardin target genes
CArG-SNP discovery and activity
LncRNA discovery and function
Development of new mouse models and correction of Mendelian traits in utero using Prime editing
Development of new rat models to interrogate the SRF-Myocardin switch under various stress conditions (eg, exercise and disease models)
JagWire: PRIME EDITING IN MICE DEFINES CRITICAL ROLE FOR A SINGLE BASE IN SMC-SPECIFIC GENE EXPRESSION
MCG MAGAZINE: Bacterial Lessons
DR. MIANO'S RESEARCH IMAGES FEATURED ON JOURNAL COVERS
INKILN is a novel long noncoding RNA promoting vascular smooth muscle inflammation via scaffolding MKL1 and USP10. bioRxiv. 2023 Jan 9:2023.01.07.522948. doi: 10.1101/2023.01.07.522948. Preprint.PMID: 36711681
PGC-1α senses the CBC of pre-mRNA to dictate the fate of promoter-proximally paused RNAPII. Mol Cell. 2023 Jan 19;83(2):186-202.e11. doi: 10.1016/j.molcel.2022.12.022.PMID: 36669479
Generation and Comparative Analysis of an Itga8-CreER T2 Mouse with Preferential Activity in Vascular Smooth Muscle Cells. Nat Cardiovasc Res. 2022 Nov;1(11):1084-1100. doi: 10.1038/s44161-022-00162-1. Epub 2022 Nov 11.PMID: 36424917
Mediterranean G6PD variant rats are protected from Angiotensin II-induced hypertension
and kidney damage, but not from inflammation and arterial stiffness. Vascul Pharmacol. 2022 Aug;145:107002. doi: 10.1016/j.vph.2022.107002.
Epub 2022 May 24.PMID: 35623546
Of mice and human-specific long noncoding RNAs. Mamm Genome. 2022 Jun;33(2):281-292. doi: 10.1007/s00335-022-09943-2.
Epub 2022 Feb 1.PMID: 35106622