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Research Interests

The Miano lab focuses primarily on the SRF-Myocardin transcriptional switch.  CRISPR/Prime Editing platforms are used extensively in rodent models to interrogate the regulation and function of SRF-Myocardin, and its downstream targets, in disease models.  The long-term goals are to (a) understand the nature of the SRF-Myocardin switch and variations in the sequences it binds under a variety of pathobiological contexts and (b) develop strategies for the diagnosis and treatment of diseases associated with SRF-Myocardin and its targets.




Research Projects

Miano Lab Summary

Transcriptional/post-transcriptional regulation of the SRF-Myocardin switch
Function and regulation of novel SRF-Myocardin target genes
CArG-SNP discovery and activity
LncRNA discovery and function
Development of new mouse models and correction of Mendelian traits in utero using Prime editing
Development of new rat models to interrogate the SRF-Myocardin switch under various stress conditions (eg, exercise and disease models)




Spotlight Publications

JagWire: PRIME EDITING IN MICE DEFINES CRITICAL ROLE FOR A SINGLE BASE IN SMC-SPECIFIC GENE EXPRESSION

MCG MAGAZINE: Bacterial Lessons

DR. MIANO'S RESEARCH IMAGES FEATURED ON JOURNAL COVERS


 

Recent Publications

Gao P, Lyu Q, Ghanam AR, Lazzarotto CR, Newby GA, Zhang W, Choi M, Slivano OJ, Holden K, Walker JA, Kadina AP, Munroe RJ, Abratte CM, Schimenti JC, Liu DR, Tsai SQ, Long X, Miano JM.  Prime editing in mice reveals the essentiality of a single base in driving tissue-specific gene expression.  Genome Biol. 16;22(1):83, 2021. PubMed ID: 33722289

Gao P, Gao P, Zhao J, Shan S, Luo W, Slivano OJ, Zhang W, Tabuchi A, LeMaire SA, Maegdefessel L, Shen YH, Miano JM, Singer HA, Long X. MKL1 cooperates with p38MAPK to promote vascular senescence, inflammation, and abdominal aortic aneurysm.  Redox Biol.  41:101903, 2021.  PubMed ID: 33667992

Kitagawa A, Kizub I, Jacob C, Michael K, D'Alessandro A, Reisz JA, Grzybowski M, Geurts AM, Rocic P, Gupte R, Miano JM, Gupte SA.  CRISPR-Mediated Single Nucleotide Polymorphism Modeling in Rats Reveals Insight Into Reduced Cardiovascular Risk Associated With Mediterranean G6PD Variant.  Hypertension.  76(2):523-532, 2020.  PubMed ID: 32507041

Nagao M, Lyu Q, Zhao Q, Wirka RC, Bagga J, Nguyen T, Cheng P, Kim JB, Pjanic M, Miano JM, Quertermous T.  Coronary Disease-Associated Gene TCF21 Inhibits Smooth Muscle Cell Differentiation by Blocking the Myocardin-Serum Response Factor Pathway.  Circ. Res.  126(4):517-529, 2020.  PubMed ID: 31815603

Lyu Q, Xu S, Lyu Y-Y, Choi M, Christie CK, Slivano OJ, Rahman A, Jin Z-G, Long X-C, Xu Y-W, Miano JM.  SENCR stabilizes vascular endothelial cell adherens junction through interaction with CKAP4.  Proc. Natl. Acad. Sci., USA., 116(2):546-555, 2019.  PubMed ID: 30584103

Miano JM, Long X, Lyu Q.  CRISPR links to long noncoding function in mice: a practical approach. Vasc. Pharmacol. 14:1-12, 2019.  PubMed ID: 30822570

Gurumurthy CB, O’Brien AR, Quadros RM, Adams Jr J, Alcaide P, Ayabe S, Ballard J, Batra SK, Beauchamp M-C, Becker KA, Bernas G, Brough D, Carrillo-Salinas F, Chan W, Chen H, Dawson R, DeMambro V, D’Hont J, Dibb K, Eudy JD, Gan L, Gao J, Gonzales A, Guntur A, Guo H, Harms DW, Harrington A, Hentges KE, Humphreys N, Imai S, Ishii H, Iwama M, Jonasch E, Karolak M, Keavney B, Khin N-C, Konno M, Kotani Y, Kunihiro Y, Lakshmanan I, Larochelle C, Lawrence CB, Li L, Lindner V, Liu X-D, Lopez-Castejon G, Loudon A, Lowe J, Jerome-Majewska LA, Matsusaka T, Miura H, Miyasaka Y, Morpurgo B, Moty K, Nabeshima Y, Nakade K, Nakashiba T, Nakashima K, Obata Y, Ogiwara S, Ouellet M, Oxburgh L, Piltz S, Pinz I, Ponnusamy MP, Ray D, Redder RJ, Rosen CJ, Ross N, Ruhe MT, Ryzhova L, Salvador AM, Alam SS, Sedlacek R, Sharma K, Smith C, Staes K, Starrs L, Sugiyama F, Takahashi S, Tanaka T, Trafford A, Uno Y, Vanhoutte L, Vanrockeghem F, Willis BJ, Wright CS, Yamauchi Y, Yi X, Yoshimi K, Zhang X, Zhang Y, Ohtsuka M, Das S, Garry DJ, Hochepied T, Thomas P, Parker-Thornburg J, Adamson AD,  Yoshiki A, Schmouth J-F, Golovko A, Thompson WR, Lloyd KCK, Wood JA, Cowan M, Mashimo T, Mizuno S, Zhu H, Kasparek P, Liaw L, Miano JM, Burgio G. Reproducibility of CRISPR-Cas9 methods for generation of conditional mouse alleles: a multi-center evaluation. Genome Biol. Aug 26; 20(1):171, 2019.  PubMed ID: 31446895

PubMed