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Research Description

Our laboratory explores multiple aspects of cardiovascular cell biology as they relate to vascular dysfunction and cardiovascular disease.

We employ transgenic and knockout mouse models, ex vivo human and murine vascular specimens, in vitro cell culture systems, fluorescent and high resolution imaging techniques, and a wide variety of other molecular and biochemical tools to better understand the mechanisms associated with vascular pathologies. Current projects are examining the signaling mechanisms regulating macropinocytosis in leukocytes and vascular cells and to investigate the role of scavenger receptor-independent internalization of cholesterol by macrophages in the pathomechanism of atherosclerosis. Another area of major focus is defining the mechanisms that mediate lipoprotein efflux from atherosclerotic arteries via the lymphatic vasculature and induce plaque regression. Additional projects include identification of novel mechanisms of entry for circulating LDL into and across the endothelium and intercellular communication via macropinocytosis in the pathogenesis of aneurysm formation.


1. Visualization of macrophage fluid-phase macropinocytosis

Figure 1

Antioxid Redox Signal (2017) PMID: 27488058

2. Thrombospondin 1 promotes macropinocytosis of native LDL in macrophages:

Fig 2

Antioxid Redox Signal (2017) PMID: 27958762

 

3. Development of new pharmacological and genetic tools to more fully explore the role of macropinocytosis in pathological processes.

 Fig 3

Br J Pharmacol (2018) PMID: 29953580

4. Identification of downstream effectors and cellular mechanisms by which neurofibromin 1 (NF1) deficiency contributes to cardiovascular disease. 

fig 5

 

Redox Biol (2019) PMID: 31201114

5. Role of R-spondin 2 in arterial lymphangiogenesis and atherosclerosis:

                     Human coronary arteries

fig. 6  

Cardiovasc Res (2020) PMID: 32750106

                             

 




Spotlight Publications

ARS Discoveries Journal Cover- Volume 24, Number 16, June 7, 2017- Antioxidants & Redox Signaling- Macropinocytosis in macrophages

Macropinocytosis in macrophages

 

CD47 and Nox1 Mediate Dynamic Fluid-Phase Macropinocytosis of Native LDL
Csányi G, Feck DM, Ghoshal P, Singla B, Lin HP, Nagarajan S, Meijles DN, Al Ghouleh I, Cantu-Medellin NC, Kelley EE, Mateuszuk LM, Isenberg JS, Watkins SC, and Pagano PJ. Antioxidants & Redox Signaling. 26:886-901 (2017).
Antioxidants & Redox Signaling (ARS), June 1, 2017    

  FULL ARTICLE   

 


 

 


    

Recent Publications

Singla B, Lin HP, Ahn W, Xu J, Ma Q, Sghayyer M, Dong K, Cherian-Shaw M, Zhou J, Huo Y, White J, Csányi G. Loss of myeloid cell-specific SIRPα, but not CD47, attenuates inflammation and suppresses atherosclerosis. Cardiovasc Res2022 Dec 9;118(15):3097-3111doi: 10.1093/cvr/cvab369. PubMed PMID: 34940829; PubMed Central PMCID: PMC9732525.

Lin HP, Singla B, Ahn W, Ghoshal P, Blahove M, Cherian-Shaw M, Chen A, Haller A, Hui DY, Dong K, Zhou J, White J, Stranahan AM, Jasztal A, Lucas R, Stansfield BK, Fulton D, Chlopicki S, Csányi G. Receptor-independent fluid-phase macropinocytosis promotes arterial foam cell formation and atherosclerosis. Sci Transl Med2022 Sep 21;14(663):eadd2376doi: 10.1126/scitranslmed.add2376. Epub 2022 Sep 21. PubMed PMID: 36130017; PubMed Central PMCID: PMC9645012.

 

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