While much more of a laboratory scientist than a field biologist, I did have a Madagascan
Hissing Cockroach as a pet in college. Its name was Bug.
And now for a fact more relevant to my research: Of the roughly 400,000 ovarian follicles present at puberty, fewer than 400 will actually reach the final stage of development and ovulate.
My background is in female reproductive physiology. My doctoral and postdoctoral research involved characterizing the proliferation and differentiation of ovarian granulosa cells, the cells that make estrogen and progesterone in the female ovary. Upon joining the Department of Biological Sciences, I wanted to merge my interests in reproductive physiology with the environmental focus of the department. We started examining how certain environmental factors, such as the perfluorinated compounds found in many consumer products like fast food packaging and water- and flame-resistant clothing and other materials, affect steroidogenic cells as well as steroid-responsive cells. In addition, I am doing collaborative work with Dr. Reichmuth looking at markers of endocrine disruption in crabs of polluted vs. non-polluted beaches. Some of the techniques used in my lab include tissue culture, RT-PCR, ELISAs, and western blotting.
Effects of PFOA on MCF-7 breast cancer cells
Current Student: Laura Gillen, Rachel Peloquin
Former Student: Amber Colquitt, April Smith
Endocrine Disruption of Marine Benthic Crustaceans (joint project with Dr. Reichmuth)
Current Student: Jennifer Mercer
Effects of PFOA on steroidogenesis and gene expression in mouse Leydig tumor (mLTC-1)
Former Students: Sandra Tadros, Ebony Miller, Latasha Lawson, Mark Yassa
Wren A, Ridlehoover M, Minter Z, Cannon JD (2013). “The Role of Insulin in Maintaining Blood Glucose Homeostasis: A Lesson Plan Used in Conjunction with the video ''Hillbilly Hypoglycemia'' Life Science Teaching Resource Community (formerly American Physiological Society Archive of Teaching Resources).
Cannon JD, Seekallu S, Vandervoort CA, Chaffin CL (2009). "Association of Luteinizing Hormone
Receptor Gene Expression with Cell Cycle Progression in Granulosa Cells." American Journal of Physiology. Endocrinology and Metabolism 296(6):E1392-9.
Cannon, JD, Cherian-Shaw M, Lovekamp-Swan T, Chaffin CL (2007). "Granulosa cell expression of G1/S phase cyclins and cyclin-dependent kinases in PMSG-induced follicle growth." Molecular and Cellular Endocrinology 264: 6-15.
Cannon JD, Cherian-Shaw M, Chaffin CL (2005). "Proliferation of rat granulosa cells during the periovulatory interval." Endocrinology 146(1): 414-22.
Chaffin CL and Cannon JD. (2005). Follicle Growth and Ovulation. In: Frontiers in Reproductive Endocrinology: A Comprehensive Review and Update. Serona Symposia International, Inc.; 109-114
Miller EL, Tadros SY, and Cannon JD: Perfluorooctanoic Acid-Induced Progesterone Synthesis Inhibition is Associated with Decreased Expression of Steroidogenic Acute Regulatory Protein (StAR) mRNA but Not p450 Side-Chain Cleavage mRNA in hCG-Stimulated mLTC-1 Cells (Poster), Experimental Biology National Meeting, Boston, March 2015
Smith AN and Cannon JD: The effects of Perfluorooctanoic acid on cell viability and peroxisome proliferator-activated receptor gene expression in MCF-7 Cells
Georgia Academy of Science Annual Meeting, Milledgeville, GA, March 2015 (Platform)
Phi Kappa Phi Research Conference, Augusta, GA, March 2015(Poster) *
Beta Beta Beta Southeastern Region Annual Meeting, Chattanooga, TN, April 2015 (Platform)#
*Awarded Best Overall Poster at PKP Research Conference
# Awarded 3rd Place for Undergraduate Oral Presentations
Yassa M, Lawson L, and Cannon JD: Comparison of Four Commercially Available RNA Extraction Products (Poster), Phi Kappa Phi Research Conference, March 2015
Cannon JD, Mercer J, and Reichmuth J: Vitellogenin expression and morphometrics in two populations of fiddler crab in the southeastern United States, Southeastern Estuarine Research Society Spring Meeting, Jacksonville, FL, March 2015
Tadros SY and Cannon JD: Perfluorooctanoic acid induced reduction in steroidogenesis in mLTC-1 cells: is it due to downregulation of the luteinizing hormone receptor? (Platform) Beta Beta Beta Southeastern Region Annual Meeting, Spartanburg, SC, April 2014
Mercer J, Cannon JD, and Reichmuth J: Vitellogenin expression and morphometrics in two populations of fiddler crab in the southeastern United States (Poster) Phi Kappa Phi Research Conference, Augusta University, Augusta, GA, March 2014
Tadros SY and Cannon JD: Is luteinizing hormone receptor (LHR) reduced in mouse leydig tumor cells treated
with PFOA? (Poster)
-Georgia Academy of Science Annual Meeting, Augusta, GA, March 2014 and
-Phi Kappa Phi Research Conference, Augusta University, Augusta, GA March 2014
Tadros SY and Cannon JD: Perfluorooctanoic acid (PFOA) decreases steroidogenesis in mouse leydig tumor cells (Poster)*
-Georgia Academy of Science Annual Meeting, Valdosta, GA, March 2013 and
-Phi Kappa Phi Research Conference, Augusta University, Augusta, GA March 2013
*Awarded Best Interdisciplinary Poster at PKP Research Conference
Endocrinology students win the American Physiology Society’s Phantastic Physiology Voyage: “Function Follows Form” Video Contest. Experimental Biology Meeting, Boston, MA, April 2013.
Phi Kappa Phi Research Conference, Augusta, GA, March 2015