The Nuclear Magnetic Resonance (NMR) instruments can provide structure or molecular interaction analysis of small organic molecules, peptides, 3D structure of proteins up to 12-15 kDa, ligand binding to larger proteins, lipids, carbohydrates, nucleic acids of 8 kDa or less, and selected metals.
The 500 MHz NMR instrument has different probes allowing multiple configurations while the 300 MHz NMR is limited to proton, carbon, and fluorine studies.
Trained users have 24h access.
Consultation is available to determine recommended experiments, to operate the instrument, or to assist with data interpretation. Long experiments are frequently restricted to nights and weekends. Experiments that require a hardware change must be scheduled in advance.
Default hardware includes a Prodigy Cryoprobe (Inverse probe) for high sensitivity proton, carbon, nitrogen, and fluorine studies of samples in liquid solution. Available hardware includes a High Resolution Magic Angle Spinning probe for lipids, cells, polymers, or high viscosity samples and a Broad Band (Direct probe) for a wide range of nuclei.
Contact facility to arrange analysis of external or commercial samples. Training is available for those who wish to become independent users.
|Protein folding||Ionization state||Solution structure|
|Molecular dynamics||Protein hydration||Hydrogen bonding|
|Metabolite analysis||Chemical analysis||Material science|
|Drug screening and design||Native membrane protein||Weak intermolecular interactions|
|Console||Bruker Avance||Bruker Avance III|
|Standard Probe||Quattro Nucleus Probe (QNP)||Triple Resonance Cryoprobe (H1, C13, N15, F19)|
|Other probes, hardware||Broad Band probe (BBP) and High resolution magic angle spinning probe, 24-tube autosampler|
GENERAL SAFETY GUIDELINES
Person with electronic, electrical or mechanical medical implants (such as cardiac implants, pacemarkers, aneurysm clips, surgical clips or prostheses…) MUST remain outside the NMR laboratories and check with the director before entering the NMR room. -Do NOT bring any metallic object within 10 feet of any magnet
-Do NOT bring compressed gas cylinders into the NMR laboratories without NRM personnel supervision
-All magnetic objects should be kept outside the NMR magnet room. This includes keys, tools, or mechanical watch, etc. ASSUME any piece of METAL is magnetic until proven otherwise.
-Keep electronics, credit cards and magnetic storage media outside of the NMR magnet room.
-NEVER put any object into the magnet, except the NRM tube and holder
-In case of a metallic object strikes the magnet, get facility staff IMMEDIATELY. Do NOT attempt to pull the object off yourself.
-In the event of a magnet quench (sudden and rapid boil-off the magnets cryogens (helium and nitrogen) that can be detected by visible (and/or audible) emission of cryogenic gas from the magnet), the magnet cryogens that vaporizes displace air and can result in asphyxiation. If a quench occurs, IMMEDIATELY EVACUATE THE NMR ROOM!
-For low-temperature NMR experiments, use the non-magnetic nitrogen dewar that is available. Do not use a wrench to disconnect the quick connector on the dewar.
-All users must follow the general rules of the Chemical and Biomolecular Analysis Facility
-No sample preparation in the NMR lab
-Do not leave your NMR tubes in the NMR spectrometer
-If a sample breaks in the magnet, contact the director immediately so the probe can be cleaned.
-All users must reserve the spectrometer using the iLab.
-All users must sign the spectrometer log-book
-Non-standard experiments/probe change must be scheduled with the director in advance.
INSTRUCTIONS AND MANUALS
Thomas D. Crute
Professor of Chemistry
Area of interest: Organic Chemistry
Assistant professor of Chemistry
Area of interest: Bioorganic & Medicinal Chemistry
Associate professor of Chemistry
Area of interest: Organometallic Chemistry