CyTOF & High-Dimensional Cytometry Core
The Georgia Cancer Center CyTOF & High-Dimensional Cytometry Core (RRID: SCR_025747) bridges the gap between the cutting-edge flow cytometry technologies and your research goals.
We provide end-to-end solutions from experimental and panel design to advanced high-dimensional data analysis.
Mass cytometry, or CyTOF (cytometry by time-of-flight), integrates the principles of flow cytometry with the quantitative capabilities of mass spectrometry, enabling multi-parametric single-cell analysis with exceptional throughput and resolution. By employing metal isotopes for antibody labeling, mass cytometry theoretically provides up to 140 detection channels with minimal signal spillover between channels.
CyTOF-XT from Multiplex Bio(formerly Standard Biotools)
has been in operation at the core since 2022.
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Mass Cytometry
Health Sciences Campus
GCC - M. Bert Storey Research Building
CN4212, CN3140
The Breakthrough Single-Cell Technology
| FLUORESCENCE FLOW CYTOMETRY | VS. |
MASS CYTOMETRY (CyTOF) |
| Broad, overlapping emission spectra | Signal Generation | Discrete metal isotope masses |
| Compensation or spectral unmixing required | Data Processing | No compensation |
| Spillover spreading error | Data Quality | Minimal channel overlap |
| Affected by autofluorescence | Background | No autofluorescence |
| Tandem dye instability | Reagent Stability | Stable metal isotopes |
| Limited multiplexing (10-45 parameters typical) | Multiplexing Capacity | 50+ markers routinely |
| Complex panel design and optimization | Panel Design | More straightforward panel design |
| Live-cell sorting possible | Cell Recovery | Cells are destroyed during analysis |
| Fast acquisition (high event rate) | Acquisition Speed | Slower acquisition (lower event rate) |
Optimized Ready-to-Go panels for human and mouse samples, easily customizable
And Many More in Core Ab bank (list of antibodies)
Your One-Stop Solution
Listing of services provided
Pricing
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- Core Abs: $7/ul;
- Human MDIPA kit (30-Ab tube): $300/unit
- Barcoding: Pd-$50/sample
- CD45 or TeMal- $7/ul
- Ab conjugation kit: $150-$175/Rxn
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- Staff-operated CyTOF-XT usage: $100/hr
- Technical assistance: $75/hr
- Initial consultation, Letter of Support: FREE
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