Core Faculty

Alexander D. Verin, PhD.

Alexander D. Verin, PhD


Department of Medicine, Pulmonary 


Research Interests

Biochemical basis of endothelial (EC) cytoskeletal regulation and permeability

  • Structure and function of contractile proteins and protein phosphatases

  • Phosphorylation/
    dephosphorylation as mechanism regulation of EC contractility

  • Relationship between EC contractility/cytoskeleton and barrier function

  • Crosstalk between microtubules and microfilaments in EC barrier regulation

  • Contractile responses to bioactive mediators and receptor/signal coupling in EC

Current Projects

  • Microtubules in endothelial cell barrier regulation.
  • Ser/Thr phosphatases in endothelial cell barrier regulation.
  • Molecular mechanisms of TGF�-induced EC permeability.
  • Molecular mechanisms of ATP-induced EC barrier enhancement.

Active Grant Support

NIH HL67307 - Microtubules in lung endothelial cell barrier regulation.
4 yrs (2006-2010), $250,000/year,
Principal Investigator.

The major goals of this project is to examine the role of G-proteins and microtubule (MT)-binding proteins in thrombin-induced MT remodeling and barrier failure. In addition, we propose to examine the role of MT remodeling in sepsis-induced lung injury in vivo.

NIH HL083327 - "ATP in lung endothelial barrier enhancement".
4 yrs (2006-2010), $250,000/year,
Principal Investigator.

The major goal of this project is to examine molecular mechanisms of ATP-induced endothelial cell barrier protection in vitro and in murine model of acute lung injury.

NIH HL080675 - "ALK/SMAD signaling in TGF-�-induced EC permeability".
4 years (2005-2009), $250,000/year,
Principal Investigator.

The major goal of this project is to examine molecular mechanisms of TGF-�-induced endothelial cell barrier dysfunction.

NIH HL058064 - Program Project "Cytoskeletal regulation of lung endothelial pathobiology"
5 yr (2003-2008),
(Principal Investigator Garcia JGN)

Project 5, "Microtubule involvement in endothelial pathobiology",
Project Leader.

The major goals of this project are to examine the role of calmodulin-dependent enzymes in MT remodeling and role of MT dissolution in endothelial cell contraction and permeability.

Honors and Awards

  • 1994 - American Federation of Medical Research Trainee Award.
  • 1992-1994 - Indiana University Dean's Council Member.
  • 1990 - Moscow State University School of Biology Young Scientists Committee Award

Editorial Board

  • 2006-present: American Journal of Physiology (Lung)

Current and Past Trainees

Joyce Gonzales, MD. 2007-present. Pulmonary Fellow.

Zhenghong Fan, MD. 2007-present. Research Associate.

Agnieszka Jezierska, PhD. 2007-present. Postdoctoral Fellow.

Umapathy Siddaramappa, PhD. 2007-present. Research Associate.

Alexander Antonov, PhD. 2006-2007. Postdoctoral Fellow.

Istvan Csikora, MS. 2006 - 2007. Visiting PhD Student.

Kyung-mi Kim, PhD. 2006 - 2007. Postdoctoral Fellow.

Dimitry Gryadunov, PhD. 2004. Postdoctoral Fellow.

Gabor Olah, MS. 2003-2005 Trainee. 2005 -2006 Visiting PhD Student.

Irina Zakeeva, MS. 2003-2004. Trainee.

Kseniya Smurova, PhD. 2002-2003. Recipient of FEBS stipend 2005.

risztina Tar, PhD. 2002-2003. Trainee. Featured speaker on FASEB Experimental biology 2004 "Graduate Student Highlights in Respiration Physiology" session. 2005-2007 Postdoctoral Fellow.

Janibek Adyshev, PhD. 2001-2005. Postdoctoral Fellow.

Irina Kolosova, PhD. 2001-2005. Research Associate. 2004 Recipient of ALA Grant-in-Aid of Maryland.

Tamara Mirzopoyazova, PhD. 2001-2005. Postdoctoral Fellow. Featured speaker on FASEB Experimental Biology 2004 "Hot Topics in Lung Endothelial Biology" session.

Anna Birukova, MD. 2003-2005. Research Associate. 2005 Recipient of AHA National Scientist Development Grant. 2005 CSCR best poster presentation award.

Talaibek Borbiev, PhD. 2001-2004. Research Associate. 2001 National AFMR Trainee Award.

Natalia Bogatcheva, PhD. 2000-2001. Postdoctoral Fellow. 2001 Midwest AFMR Trainee Award.


(from 90 total)

Verin AD, Gusev NB. Ca2+-induced conformational changes in cardiac troponin C as measured by N-(1- Pyrene) maleimide fluorescence. Biochem Biophys Acta, 1988; 956:197-208.

Verin AD, Patterson CE, Day MA, Garcia JGN. Regulation of endothelial gap formation and barrier function by myosin-associated phosphatase activities. Am. J. Physiol.(Lung Cell. Mol. Physiol), 1995; 269:L99-L108.

Garcia JGN, Lazar V, Gilbert McClain LI, Gallagher PJ, Verin AD. Myosin light chain kinase in endothelium: Molecular cloning and regulation. Am. J. Respir. Cell Mol. Biol., 1997; 16: 489-494.

Verin AD, Cooke C, Herenyova M, Patterson CE, Garcia JGN. Role of Ca2+/ calmodulin-dependent phosphatase 2B in thrombin-induced endothelial cell contractile response. Am. J. Physiol. (Lung Cell. Mol. Physiol), 1998; 275: L788-L799.

Verin AD, Lazar V, Torry RJ, Labarrere CA, Patterson CE, Garcia JGN. Expression of a novel high molecular weight myosin light chain kinase in endothelium. Am. J. Respir. Cell Mol. Biol., 1998; 19: 758-766.

Verin AD, Gilbert-McClain LI, Patterson CE, Garcia JGN. Biochemical regulation of the non-muscle myosin light chain kinase isoform in bovine endothelium. Am. J. Respir. Cell Mol. Biol., 1998; 19: 767-776.

Verin AD, Wang P, Garcia JGN. Immunochemical characterization of myosin-specific phosphatase 1 regulatory subunits in bovine endothelium J. Cell. Biochem., 2000; 76: 489-498.

Verin AD, Liu F, Bogatcheva NV, Borbiev T, Hershenson MB, Wang P, Garcia JGN. Role of Ras-dependent ERK activation in phorbol ester-induced endothelial cell barrier dysfunction. Am. J. Physiol. (Lung Cell. Mol. Physiol), 2000; 279: L360-L370.

Verin AD, Csortos C, Durbin SD, Wang P, Aydonyan A, Patterson CE, Garcia JGN. Characterization of the protein phosphatase 1 catalytic subunit in endothelium: involvement in contractile responses. J. Cell. Biochem., 2000; 79: 113-125.

Garcia JGN, Wang P, Liu F, Hershenson MB, Borbiev T, Verin AD Pertussis toxin directly activates endothelial cell p42/p44 MAP kinases via a novel signaling pathway Am. J. Physiol. (Cell Physiol), 2001; 280: C1233-C1241.

 Birukov K, Csortos C, Marzilli L, Dudek S, Ma SF, Bresnik A, Verin AD, Cotter RJ, Garcia JGN. Differential regulation of alternatively spliced endothelial cell myosin light chain kinase isoforms by p60src. J. Biol. Chem., 2001; 276: 8567-8573.

Verin AD, Birukova A., Wang P, Birukov K, Liu F, Garcia JGN. Microtubule disassembly increases endothelial cell barrier dysfunction: role of myosin light chain phosphorylation. Am. J. Physiol. (Lung Cell. Mol. Physiol), 2001; 281: L565-L574.

Garcia JGN, Liu F, Verin AD, Birukova A, Dechert MA, Gerthoffer WT, Bamburg J, English DK. Sphingosine-1-phosphate promotes endothelial cell barrier integrity by Edg-dependent cytoskeletal rearrangement. J. Clin. Invest., 2001; 108: 689-701. (Cover).

Bogatcheva NV, Garcia JGN, Verin AD. Molecular mechanisms of thrombin-induced endothelial cell permeability. Biochemistry (Moscow) 2002; 67: 75-84. (Invited Review).

Garcia JGN, Wang P, Schaphorst KL, Becker P, Borbiev T, Liu F, Birukova A, Jacobs K, Bogatcheva N, Verin AD. Critical involvement of p38 MAP kinase in pertussis toxin-induced cytoskeletal reorganization and lung permeability. FASEB J, 2002; 16: 1064-1076.

Liu F, Schaphorst KL, Verin AD, Jacobs KN, Birukova A, Day RM, Bogatcheva NV, Bottaro DP, Garcia JGN. Hepatocyte growth factor enhances endothelial barrier function and cortical cytoskeletal rearrangement: potential role of glycogen synthase kinase 3B. FASEB J, 2002; 16: 950-962.

Bogatcheva NV, Garcia JGN, Verin AD. Role of tyrosine kinase signaling in endothelial cell barrier regulation. Vascul. Pharmacol. 2002; 39:201-12. (Invited Review).

Petrache I, Birukova A, Ramirez SI, Garcia JGN, Verin AD. The role of the microtubules in TNF-a?-induced endothelial cell permeability. Am J Respir Cell Mol Biol. 2003; 5: 574-581.

Kolosova I, Csortos C, Garcia JGN, Verin AD. Role of Ser/Thr protein phosphatases in endothelial cytoskeletal organization and barrier function. Recent Res. Devel. Physiol., 1(2003): 385-400. (Invited Review).

Bogatcheva NV, Dudek SM, Garcia JGN, Verin AD. MAP kinases in endothelial pathophysiology. J Investig Med. 2003 Nov; 51(6):341-52. (Review).

Shikata, Y, Birukov KG, Birukova AA, Verin AD, Garcia JGN. Involvement of site-specific FAK phosphorylation in sphingosine-1 phosphate- and thrombin-induced focal adhesion remodeling: role of Src, and GIT. FASEB J. 2003 Dec; 17(15):2240-9.

Birukova AA, Smurova K, Birukov KG, Kaibuchi K, Garcia JGN, Verin AD. Role of Rho GTPases in thrombin-induced lung vascular endothelial cells barrier dysfunction. Microvasc Res. 2004 Jan; 67(1):64-77.

Regard JB, Scheek S, Borbiev T, Lanahan AA, Schneider A, Demetriades AM, Hiemisch H, Barnes CA, Verin AD, Worley PF. Verge: a novel vascular early response gene. J Neurosci. 2004 Apr 21; 24(16):4092-103.

Tar K, Birukova AA, Csortos C, Bako E, Garcia JGN, Verin AD. Phosphatase 2A involves in endothelial cell microtubule remodeling and barrier regulation. J Cell Biochem. 2004 Jun 1; 92(3):534-46.

Birukova AA, Liu F, Garcia JG, Verin AD. Protein kinase A attenuates endothelial cell barrier dysfunction induced by microtubule disassembly. Am J Physiol Lung Cell Mol Physiol. 2004 Jul; 287(1):L86-93.

Birukova AA, Smurova K, Birukov KG, Usatiuk P, Liu F, Kaibuchi K, Ricks-Cord A, Natarajan V, Alieva I, Garcia JGN, Verin AD. Microtubule disassembly induces cytoskeletal remodeling and lung vascular barrier dysfunction: Role of Rho-dependent mechanisms. J Cell Physiol. 2004 Oct; 201(1):55-70. (Cover).

Borbiev T, Birukova A, Liu F, Nurmukhambetova S, Gerthoffer WT, Garcia JGN, Verin AD. P38 MAP kinase-dependent regulation of endothelial permeability. Am J Physiol Lung Cell Mol Physiol 2004;287 L911-L918.

Kolosova IA, Ma S-F, Adyshev DM, Wang P, Ohba M, Natarajan V, Garcia JGN, Verin AD. The role of CPI-17 in the regulation of endothelial cytoskeleton. Am J Physiol Lung Cell Mol Physiol. 2004 Nov; 287(5):L970-80.

Birukov KG, Bochkov VN, Birukova AA, Kawkitinarong K, Rios A, Leitner A, Verin AD, Bokoch GM, Leitinger N, Garcia JG. Epoxycyclopentenone-Containing Oxidized Phospholipids Restore Endothelial Barrier Function via Cdc42 and Rac. Circ Res. 2004 Oct 29; 95(9):892-901.

Birukova AA, Birukov KG, Smurova K, Adyshev D, Kaibuchi K, Alieva I, Garcia JGN, Verin AD. Novel role of G-proteins and microtubules in thrombin-induced endothelial barrier dysfunction FASEB J. 2004 Dec; 18 (15):1879-90.

Mirzapoiazova T, Kolosova IA, Romer L, Garcia JGN, Verin AD. The role of caldesmon in the regulation of endothelial cytoskeleton and migration. J Cell Physiol. 2005 Jun; 203(3):520-8.

Verin AD. Tyrosine phosphorylation and endothelial cell barrier regulation. Am J Pathol. 2005 Apr; 166(4):955-7. (Invited Review).

Bogatcheva NV, Sergeeva M, Dudek S, Verin AD. Arachidonic acid cascade in the regulation of vascular endothelial permeability. Microvasc Res. 2005 May; 69(3):107-27. (Review).

Teckchandani AM, Birukova AA, Tar K, Verin AD, Tsygankov AY. The multidomain protooncogenic protein c-Cbl binds to and stabilizes microtubules. Exp Cell Res. 2005 May 15; 306(1):114-27.

Birukova AA, Birukov KG, Adyshev D, Usatyuk P, Natarajan V, Garcia JGN, Verin AD Involvement of microtubules and Rho pathway in TGF-�1-induced lung vascular barrier dysfunction. J Cell Physiol. 2005 Sep; 204(3):934-47.

Becker PM, Waltenberger J, Yachechko R, Mirzapoiazova T, Sham JS, Lee CG, Elias JA, Verin AD. Neuropilin-1 regulates vascular endothelial growth factor-mediated endothelial permeability. Circ Res. 2005 Jun 24; 96(12):1257-65.

Birukova AA, Adyshev D, Gorshkov B, Birukov KG, Verin AD. ALK5 and Smad4 are involved in TGF-beta1-induced pulmonary endothelial permeability. FEBS Lett. 2005 Jul 18; 579(18):4031-7.

Kolosova IA, Mirzapoiazova T, Adyshev D, Usatyuk P, Romer LH, Jacobson JR, Natarajan V, Pearse DB, Garcia JG, Verin AD. Signaling pathways involved in adenosine triphosphate-induced endothelial cell barrier enhancement. Circ Res. 2005 Jul 22; 97(2):115-24.

Birukova A, Birukov KG, Gorshkov B, Liu F, Garcia JGN, Verin AD, MAP kinases in lung endothelial permeability induced by microtubule disassembly. Am J Physiol Lung Cell Mol Physiol. 2005 Jul; 289(1):L75-84.

Petrache I, Birukova A, Verin AD. TNF-a-induced pulmonary endothelial permeability: the role of the microtubule cytoskeleton. "Microvascular Research: Biology and Pathology", Elsevier Ltd, D. Shepro Editor-in-Chief, 2005 Chapter 40, 261-264 (Invited Review).

Birukova AA, Adyshev D, Gorshkov B, Bokoch G, Birukov KG, Verin AD. GEF-H1 is involved in agonist-induced human pulmonary endothelial barrier dysfunction. Am J Physiol Lung Cell Mol Physiol. 2006 Mar; 290(3):L540-8.

Adyshev DM, Kolosova IA, Verin AD. Potential protein partners for the human TIMAP revealed by bacterial two-hybrid screening. Mol Biol Rep. 2006 Jun; 33(2):83-9.

Tar K, Csortos C, Czikora I, Olah G, Ma S-F, Wadgaonkar R, Gergely P, Garcia JGN, Verin AD. The role of protein phosphatase 2A in the regulation of endothelial cell cytoskeleton structure. J Cell Biochem. 2006 Jul 1; 98(4):931-53. (Cover).

Verin AD, Bogatcheva NV. Cytoskeletal proteins. Encyclopedia of Respiratory Medicine, Elsevier Ltd, GJ Laurent, SD Shapiro Editors, 2006: 615-622. (Invited Review).

Mirzapoiazova T, Kolosova I, Usatyuk P, Natarajan V, Verin AD. Diverse effects of vascular endothelial growth factor on pulmonary endothelial barrier. Am J Physiol Lung Cell Mol Physiol. 2006 Oct; 291(4):L718-24.

Bogatcheva NV, Borbiev T, Birukova AA, Kolosova I, Liu F, Garcia JGN, Verin AD. Caldesmon as cytoskeletal target of PMA- induced endothelial cells activation. J Cell Biochem. 2006 Dec 15; 99(6):1593-605.

Bogatcheva NV, Adyshev DM, Mombetsariev B, Moldabaeva N, Verin AD. Involvement of microtubules, p38 and Rho kinases pathway in 2-methoxyestradiol-induced lung vascular barrier dysfunction. Am J Physiol Lung Cell Mol Physiol. 2007 Feb; 292(2):L487-99.

Mirzapoiazova T, Kolosova IA, Moreno L, Sammani S, Garcia JG, Verin AD. Suppression of endotoxin-induced acute lung injury by taxol (Eur Respir J. 2007 May 30; [Epub ahead of print]).

Csortos C, Kolosova I, Verin AD. Regulation of vascular endothelial cell barrier function and cytoskeleton structure by protein phosphatases of the PPP family (Am. J. Physiol (Lung), accepted with minor revision). (Invited Review).


Education and Training

Indiana University: Indianapolis, Indiana, Department of Medicine.
Postdoc, Cell Physiology. 1993-1997.

Indiana University: Indianapolis, Indiana, Department of Biochemistry and Molecular Biology.
Postdoc, Molecular Biology and Biochemistry. 1990-1993.

Moscow State University: Moscow, Russia, Department of Biochemistry.
PhD., Biochemistry. 1982-1990.

Moscow State University and Institute of Molecular Biology: Moscow, Russia,
M.S. , Biology. 1977-1982.


  • 2002 - Member, NHLBI Special Emphasis Panel: The adaptations to hypoxia.
  • 2003 - Member, NHLBI Special Emphasis Panel: Functional heterogeneity of the peripheral, pulmonary and lymphatic vessels.
  • 2003 - Member, NCI Initial Review Group: Signal transduction in time and space.
  • 2004 - Member, NHLBI Special Review Committee: Signaling of endothelial permeability and lung vascular injury.
  • 2005 - Member, NIH Vascular Bioengineering Partnership Special Emphasis Panel .
  • 2005 - Health Review Board of Ireland Research Grants.
  • 2005 - Florida Department of Health Biomedical Research Program.
  • 2006 - Ad-hoc member, NHLBI Respiratory Integrative Biology and Translational Research Study Section, Respiratory Sciences Integrated Review Group (RIBT).

Society Memberships

  • American Heart Association (Basic CV Science Council)
  • American Physiological Society (Cell & Molecular Physiology Section)
  • American Society for Cell Biology
  • American Thoracic Society
  • European Respiratory Society