photo of Sharad Purohit

Sharad Purohit

  • Course Director
  • Assistant Professor




  • Graduate Research Assistant

Term: Fall 2020

Lecture room: CA4131

Meeting time: TBA

Course materials: held in Box

Course description:

This is an informal course to introduce rotating student-researchers to common concepts and techniques used in the Center for Biotechnology and Genomic Medicine. Students will learn the theory of lab techniques in a didactic setting with some demonstrations in the laboratory and using instruments. Then, in their own time, students will be able to implement and practice these skills, at first with supervision, then eventually on their own.

The purpose of this course is for students to become familiar with many techniques and concepts, both in theory and in practice, so that they may carry these skills forward during this period of rotation research and in any other projects they choose to take on in the future. All course materials will either be found at the course Box link (provided above).


The student is expected to attend the lab daily during the rotation period.

He/She must notify the supervisor at least 24 hours in advance of potential absence. Urgent medical absences are acceptable.

CBGM Specific lab meetings/Journal Club

The student is expected to meet on Monday's immediate supervisor in afternoon.

He/She will attend CBGM Journal Club on Friday (9am-10am) as a part of this course, to familiarize students with critically analyzing primary literature and picking apart the key points in each article. These articles will be pertinent to the direction of the center and are also potentially relevant to the technique being discussed each week.

Note: The student is expected to follow AU/MCG rules on dress code for working in laboratory.


Tentative Rotation Schedule

Week of




Basic lab management tasks- autoclave, freezer cleaning, filling water.

You are required to learn and perform some of the laboratory tasks during the rotation period



Laboratory calculations for buffer/salt/ analytical preps

Calculating molarity, percentage

Calculating moles of molecules for reaction purposes



Buffers and measurements



Pipetting with single and multi-channel pipette.

Pipetting is a very basic skill, but any error in pipetting, will lead to increased variation in the results. These variations are more prominent for multichannel pipets. You will practice this skill with both multichannel and single channel pipets, till your pipetting variations are within 1%. The pipetting test requires use of an ELISA reader. This has to be done twice a day on Day 1, 2, 5, and 10. Ask for the pipetting protocol



Basic calculations using Excel: calculating mean, standard deviation, and other reproducibility measures



Cell culture- eukaryotic and prokaryotic



Basic sample prep and Mass spectrometric analysis for proteomics: This module includes total protein extraction from cell, protein concentration measurement, protein reduction and alkylation, trypsin digestion, sample clean-up, LC-MS analysis, database search and interpretation.



Basic sample prep and MS analysis for glycomics

The module includes extraction glycans from cells. The student will desalt the glycan and then methylate the sample.

The methylated glycans will then be analyzed on LC-MS.






Journal club

The students will be assigned papers to be presented on once a week. (the day is yet to be determined)