Sang-Ho Kwon, PhD

 

ABOUT OUR TEAM

We are interested in extracellular vesicles, particularly exosomes, a type of extracellular vesicles released after fusion of internal vesicles, including multivesicular endosomes and the cell surface membrane. Because extracellular vesicles encapsulate intracellular small and macromolecules, they can mirror the intracellular states of cells at the molecular levels. Further, recent data suggest these vesicles can not only play roles in the extracellular space but also serve as intercellular messengers under certain conditions. To understand how these vesicles are generated and how the released vesicles can modulate cellular states of the recipient cells, we currently probe their biogenesis and roles in epithelial growth.

OUR PROJECTS

Extracellular vesicle biogenesis in epithelial cells
-Extracellular vesicles can mirror the disease states of cells. We study how cargo proteins and RNAs are selected and loaded in/on exosomes at the molecular levels, using genome-editing, RNA-Seq, Mass-Spec, and Imaging.

Extracellular vesicles in kidney injuries
-Extracellular vesicles can modulate gene expression via their release and uptake to their target cells. We study extracellular vesicle-loaded proteins and RNAs in epithelial tubule regrowth after kidney injury, using tubules-on-dish and animal models.

Exosome toolboxes
-Though exosomes hold great potential for biomarkers and therapeutic factor delivery, we still lack effective and reliable isolation, detection, and quantitation methods. We are working on exosome toolboxes to tackle these challenges.

CURRENT TEAM MEMBERS

Leader
Sang-Ho Kwon, PhD
Assistant Professor of Cellular Biology and Anatomy

Lab Manager
Byung-Rho Lee, PhD

Graduate Student
Chang Min Lee, MS

Postdoctoral Fellow
We currently have an opening for one position

Former Lab Members
Hiroko Sonoda, PhD (Visiting Professor)
Bethany Sanstrum, PhD (PostDoc)
Augustine Song (MSP Student)
Christy Lee (MSP Student)
Chenglong Li, MD (PostDoc)

CONTACT

Sang-Ho Kwon, PhD
Department of Cellular Biology and Anatomy
Medical College of Georgia at Augusta University
Augusta, GA  30912

Postdoc applicants email to Sang-Ho with CV, two references, and a brief explanation why you are interested in our lab.

Publications

1. Kwon SH, Guggino WB
“Multiple sequences in the C-terminus of MaxiK channels are involved in expression, movement to the cell surface and apical localization”
Proc Natl Acad Sci USA, PMID: 15469924 (2004)

2. Caruso-Neves C, Kwon SH, Guggino WB
“Albumin endocytosis in proximal tubule cells is modulated by angiotensin II through AT2 receptor-mediated protein kinase B activation”
Proc Natl Acad Sci USA, PMID: 16293694 (2005)

3. Kwon SH, Pollard H, Guggino WB
“Knockdown of NHERF1 enhances degradation of temperature rescued DeltaF508 CFTR from the cell surface of human airway cells.”
Cell Physiol Biochem, PMID: 17982258 (2007)

4. Kim M, O'Brien LE, Kwon SH, Mostov KE
“STAT1 is required for redifferentiation during Madin-Darby canine kidney tubulogenesis.”
Molecular Biology of the Cell, PMID: 20861313 (2010)

5. Kwon SH, Nedvetsky PI, Mostov KE
“Transcriptional profiling identifies TNS4 function in epithelial tubulogenesis."
Current Biology, PMID: 21236678 (2011)

6. Nedvetsky PI, Kwon SH, Debnath J, Mostov KE
“Cyclic AMP regulates formation of mammary epithelial acini in vitro.”
Molecular Biology of the Cell, PMID: 22675028 (2012)

7. Tukayae DN, Kwon SH, Guggino WB
“The Gap portion of Pseudomonas aeruginosa type III secreted toxin ExoS upregulates total and surface levels of wild type CFTR.”
Cell Physiol Biochem, PMID: 23428533 (2013)

8. Kwon SH, Liu KD, Mostov KE
“Intercellular transfer of GPRC5B via exosomes drives HGF-mediated outward growth.”
Current Biology, PMID: 24412205 (2014)

9. Baek JI, Kwon SH, Zuo X, Choi SY, Kim SH, Lipschutz JH
“Dynamin binding protein (Tuba) deficiency inhibits ciliogenesis and nephrogenesis in vitro and in vivo.”
Journal of Biological Chemistry, PMID: 26895965 (2016)

10. Kwon SH*, Oh SK*, Nacke M, Mostov KE, Lipschutz JH
“CD2AP and LMAN2 regulates exosome cargo protein trafficking through the Golgi Complex."
Journal of Biological Chemistry, PMID: 27765817 (2016)

11. Sagar A, Arif E, Solanski AK, Srivastava P, Janech MG, Kim SH, Lipschutz JH, Kwon SH, Nihalani D
“Targeting Neph1 and ZO-1 protein-protein interaction in podocyte prevents podocyte injury and preserves glomerular filtration function.”
Scientific reports, PMID: 28935902 (2017)

12. Zealy RW, Fomin M, Davila S, Makowsky D, Thigpen H, McDowell CH, Cummings JC, Lee ES, Kwon SH, Min KW, Yoon JH
“Long noncoding RNA complementarity and target transcripts abundance.”
Biochim Biophys Acta Gene Regul Mech, PMID: 29421307 (2018)

13. Min KW, Zealy RW, Davila S, Fomin M, Cummings JC, Makowsky D, Mcdowell CH, Thigpen H, Hafner M, Kwon SH,
Georgescu C, Wren JD, Yoon JH
“Profiling of m6A RNA modifications identified as age-associated regulation of AGO2 mRNA stability.”
Aging Cell, PMID: 29573145 (2018)

14. Srivastava P, Solanki AK, Wolk BJ, Janech MG, Budisavjevic MN, Kwon SH, Nihalani D
“Development of a novel cell-based assay to diagnose recurrent focal and segmental Glomerulosclerosis.”
Kidney International, PMID: 30709661 (2019)

15. Arif E, Solanki A, Srivastava P, Rhman B, Tash BR, Holzman LB, Janech MG, Martin R, Knolker HJ, Fitzgibbon WR, Deng P, Budisavljevic MN, Syn WK, Wang C, Lipschutz JH, Kwon SH, Nihalani D
“The motor protein Myo1c regulates transforming growth factor—signaling and fibrosis in podocytes”
Kidney International, PMID: 31097328 (2019)

16. Wang S, Kwon SH, Su Y, Dong Z
“Stress granules are formed in renal proximal tubule cells during metabolic stress and ischemic injury for cell survival”
Am J Phyiol Renal Physiol, PMID: 31091124 (2019)

17. Sonoda H, Lee BR, Park KH, Nihalani D, Yoon JH, Ikeda M, Kwon SH
“miRNA profiling of urinary exosomes to assess the progression of acute kidney injury”
Scientific Reports, PMID: 30886169 (2019)

18. Arif E, Solanki AK, Srivastava P, Rhaman B, Fitzgibbon WR, Deng P, Budisavljevic MN, Baicu CF, Zile MR, Megyesi J, Janech MG, Kwon SH, Collier J, Schnellmann RG, Nihalani D “Mitochondria biogenesis induced by β2-adrenergic receptor agonist formoterol accelerates podocyte recovery from glomerular injury”
Kidney International, PMID: 31262488 (2019)

19. Solanski AK, Widmeier E, Arif E, Sharma S, Daga A, Srivastava P, Kwon SH, Hugo H, Nakayama M, Mann N, Majmundar AJ, Tan W, Gee HY, Sadowski CE, Rinat C, Becker-Cohen R, Bergmann C, Rosen S, Somers M, Shril S, Huber TB, Mane S, Hilderbrandt F, Nihalani D.
“Mutations in KIRREL1, a slit diaphragm component, causes steroid-resistant nephrotic syndrome”
Kidney International, PMID: 31472902 (2019)

20. Lee BR, Sanstrum BJ, Liu Y, and Kwon SH.
“Distinct role of Sirtuin 1 (SIRT1) and Sirtuin 2 (SIRT2) in inhibiting cargo-loading and release of extracellular vesicles”
Scientific Reports, PMID: 31882861 (2019)

21. Wang T, Kwon SH, Peng X, Urdy S, Lu Z, Schmitz RJ, Dalton S, Mostov KE, Zhao S.
“A Qualitative change in the transcriptome occurs after the first ell cycle and coincides with lumen establishment during MDCKII cytogenesis”
iScience, PMID: 33089114 (2020)

22. Zeng M, Wen J, Ma Z, Xiao L, Liu Y, Kwon SH, Liu Y, and Dong Z.
“Foxo1-mediated downregulation of Rab27b leads to decreased exosome secretion in diabetic kidneys”
Diabetes, PMID: 33597203 (2021)

23. Solanki AK, Arif E, Srivastava P, Furcht CM, Rahman B, Wen P, Singh A, Holzman LB, Fitzgibbon WR, Budisavljevic MN, Lobo, GP, Kwon SH, Han Z, Lazzara MJ, Lipschutz JH, and Nihalani D.
“Phosphorylation of slit diaphragm proteins NEPHRIN and NEPH1 upon binding of HGF promotes podocyte repair”
Journal of Biological Chemistry, PMID: 34391780 (2021)

24. Oh S and Kwon SH.
“Extracellular vesicles in acute kidney injury and clinical applications"
International Journal Molecular Sciences, PMID: 34445618 (2021)