Assistant Professor
Department of Cellular Biology and Anatomy
Medical College of Georgia
Augusta University
1460 Laney Walker Blvd.
Augusta, GA 30912
Office: R&E Building, CB 2820A
Office Phone: 706-721-0379
Lab: R&E Building, CB 2820
Lab Phone: 706-721-0381
Fax: 706-721-6120
envelope-o icon KKWON@augusta.edu
We are interested in extracellular vesicles, particularly exosomes, a type of extracellular vesicles released after the fusion of internal vesicles, including multivesicular endosomes and the cell surface membrane. Because extracellular vesicles encapsulate intracellular small and macromolecules, they can mirror the intracellular states of cells at the molecular levels. Further, recent data suggest these vesicles can not only play roles in extracellular space but also serve as intercellular messengers under certain conditions. To understand how these vesicles are generated and how the released vesicles can modulate the cellular states of the recipient cells, we currently probe their biogenesis and roles in epithelial growth.
Extracellular vesicle biogenesis in epithelial cells
-Extracellular vesicles can mirror the disease states of cells. We study how cargo
proteins and RNAs are selected and loaded in/on exosomes at the molecular levels,
using genome-editing, RNA-Seq, mass spectrometry, and imaging.
Extracellular vesicles in kidney injuries
-Extracellular vesicles can modulate gene expression via their release and uptake
to their target cells. We study extracellular vesicle-loaded proteins and RNAs in
epithelial tubule regrowth after kidney injury, using tubules-on-dish and animal models.
Exosome toolboxes
-Though exosomes hold great potential for biomarkers and therapeutic factor delivery,
we still lack effective and reliable isolation, detection, and functionalization methods.
We are working on exosome toolboxes to tackle these challenges.
Leader
Sang-Ho Kwon, PhD
Assistant Professor of Cellular Biology and Anatomy
Lab Manager
Byung-Rho Lee, PhD
Postdoc
Heo Dan Ph.D.
Student
Chang-Min Lee
Joyce Kim
Robin Yi
Michelle Lin
We currently have an opening for one position.
Former lab members:
Hiroko Sonoda, Ph.D. (Visiting Professor)
Bethany Sanstrum, Ph.D. (PostDoc)
Augustine Song (MSP student)
Christy Lee (MSP student)
Chenglong Li, M.D. (Postdoc)
Sang-Ho Kwon, PhD
Department of Cellular Biology and Anatomy
Medical College of Georgia at Augusta University
Augusta, GA 30912
Postdoc applicants email to Sang-Ho with CV, two references, and a brief explanation why you are interested in our lab.
“Multiple sequences in the C-terminus of MaxiK channels are involved in expression, movement to the cell surface and apical localization”
Proc Natl Acad Sci USA, PMID: 15469924 (2004)
“Albumin endocytosis in proximal tubule cells is modulated by angiotensin II through AT2 receptor-mediated protein kinase B activation”
Proc Natl Acad Sci USA, PMID: 16293694 (2005)
“Knockdown of NHERF1 enhances degradation of temperature rescued DeltaF508 CFTR from the cell surface of human airway cells”
Cell Physiol Biochem, PMID: 17982258 (2007)
“STAT1 is required for redifferentiation during Madin-Darby canine kidney tubulogenesis” Molecular Biology of the Cell, PMID: 20861313 (2010)
“Transcriptional profiling identifies TNS4 function in epithelial tubulogenesis"
Current Biology, PMID: 21236678 (2011)
“cyclic AMP regulates formation of mammary epithelial acini in vitro”
Molecular Biology of the Cell, PMID: 22675028 (2012)
“The Gap portion of Pseudomonas aeruginosa type III secreted toxin ExoS upregulates total and surface levels of wild type CFTR”
Cell Physiol Biochem, PMID: 23428533 (2013)
“Intercellular transfer of GPRC5B via exosomes drives HGF-mediated outward growth”
Current Biology, PMID: 24412205 (2014)
“Dynamin binding protein (Tuba) deficiency inhibits ciliogenesis and nephrogenesis in vitro and in vivo”
Journal of Biological Chemistry, PMID: 26895965 (2016)
“CD2AP and LMAN2 regulates exosome cargo protein trafficking through the Golgi Complex"
Journal of Biological Chemistry, PMID: 27765817 (2016)
“Targeting Neph1 and ZO-1 protein-protein interaction in podocyte prevents podocyte injury and preserves glomerular filtration function”
Scientific Reports, PMID: 28935902 (2017)
“Long noncoding RNA complementarity and target transcripts abundance”
Biochim Biophys Acta Gene Regul Mech, PMID: 29421307 (2018)
“Profiling of m6A RNA modifications identified as age-associated regulation of AGO2 mRNA stability”
Aging Cell, PMID: 29573145 (2018)
“Development of a novel cell-based assay to diagnose recurrent focal and segmental Glomerulosclerosis”
Kidney International, PMID: 30709661 (2019)
“The motor protein Myo1c regulates transforming growth factor—signaling and fibrosis in podocytes”
Kidney International, PMID: 31097328 (2019)
“Stress granules are formed in renal proximal tubule cells during metabolic stress and ischemic injury for cell survival”
Am J Phyiol Renal Physiol, PMID: 31091124 (2019)
“miRNA profiling of urinary exosomes to assess the progression of acute kidney injury”
Scientific Reports, PMID: 30886169 (2019)
“Mitochondria biogenesis induced by b2-adrenergic receptor agonist formoterol accelerates podocyte recovery from glomerular injury”
Kidney International, PMID: 31262488 (2019)
“Mutations in KIRREL1, a slit diaphragm component, causes steroid-resistant nephrotic syndrome”
Kidney International, PMID: 31472902 (2019)
“Primary cilia and the exocysts are linked to urinary extracellular vesicle production and content”
Journal of Biological Chemistry, PMID: 31694916 (2019)
“Distinct role of Sirtuin 1 (SIRT1) and Sirtuin 2 (SIRT2) in inhibiting cargo-loading and release of extracellular vesicles”
Scientific Reports, PMID: 31882861 (2019)
“A Qualitative change in the transcriptome occurs after the first cell cycle and coincides with lumen establishment during MDCKII cytogenesis”
iScience, PMID: 33089114 (2020)
“FOXO1-mediated downregulation of Rab27B leads to decreased exosome secretion in diabetic kidneys”
Diabetes, PMID: 33597203 (2021)
“Phosphorylation of slit diaphragm proteins NEHRIN and NEPH1 upon binding of HGF promotes podocyte repair”
Journal of Biological Chemistry, PMID: 34391780 (2021)
“Extracellular vesicles in acute kidney injury and clinical applications”
International Journal of Molecular Sciences, PMID: 34445618 (2021)
“Upregulation of the serine palmitoyltransferase subunit SPTLC2 by endoplasmic reticulum stress inhibits the hepatic insulin response”
Experimental and Molecular Medicine, PMID: 35513574 (2022)
*Co-corresponding author
“Differential release of extracellular vesicle tRNA from oxidative stressed renal cells and ischemic kidneys”
Scientific Reports, PMID: 35102218 (2022)
“Extracellular vesicles that herald the scarcity of oxygen”
Kidney Diseases, PMID: 35702703 (2022)
“Ascorbate peroxidase-mediated in situ labeling of proteins in secreted exosomes”
Journal of Extracellular Vesicles, PMID: 35716063 (2022)