Instrumentation


 

Flow Cytometry Analytical Instruments

The facility is equipped with two 4-color Becton Dickinson FACSCalibur flow cytometers for biological cell/particle analysis. These instruments collect information on light scatter (indicating approximate cell size and complexity of exterior and interior cell structure) and up to 4 colors of fluorescence emission. These machines can be operated a rates of up to several thousand cells per second.  (Fluorofinder fluorescent reagent resource)

BDFACSCalibur

Becton Dickinson FACSCalibur

 4-color analyzer
equipped with 2 lasers


 

10 laser - 15mW 488nm air-cooled argon-ion laser

 

20 laser - 635nm red diode laser

and 4 fluorescence detectors

 

 

FL1 = 530 +/-15nm BP

(FACSCalibur FL1 fluorochrome options/examples - FITC, Alexa Fluor 488, GFP, Cy2, Fluo-3, Calcein, CFSE, DCFH, DHR123)

 

FL2 = 585 +/-21nm BP

(FACSCalibur FL2 fluorochrome options/examples - PE, Cy3, PI)

 

FL3 = 670+nm LP

(FACSCalibur FL3 fluorochrome options/examples - PerCP, PE-Cy5, PerCP-Cy5.5, PE-Cy7, PI)

 

FL4 = 661 +/- 8nm BP

(FACSCalibur FL4 fluorochrome options/examples - APC, Alexa Fluor 647, Cy5)

 

Flow Cytometry Cell Sorter

The facility is equipped with a Beckman Coulter MoFlo XDP cell sorter that can perform up to 7 color analysis at speeds of up to 30,000 cells/second and can collect from 1 to 4 separate cell populations. (Fluorofinder fluorescent reagent resource)

MoFloCellSorter

Beckman Coulter  MoFlo XDP

 7-color Cell Sorter
equipped with 2 lasers


 

10 laser - Coherent Sapphire 488nm 200mW air-cooled argon-ion laser

 

20 laser - Coherent OBIS 640nm laser

 and 7 fluorescence detectors

 

 

FL1 = 530 +/- 20nm BP

(MoFlo XDP FL1 fluorochrome options/examples - FITC, Alexa Fluor 488, GFP Cy2 Fluo-3, Calcein, CFSE, DCFH, DHR123)

 

FL2 = 580 +/- 15nm BP

(MoFlo XDP FL2 fluorochrome options/examples - PE, Cy3, PI)

 

FL3 = 610 +/- 10nm BP

(MoFlo XDP FL3 fluorochrome options/examples - PE-Texas Red and PI)

 

FL4 = 670 +/-15nm BP

(MoFlo XDP FL4 fluorochrome options/examples- PerCP, PE-Cy5, PerCP-Cy5.5, PI)

 

FL5 = 740+nm LP

(MoFlo XDP FL5 fluorochrome options/examples - PE-Cy7)

 

FL6 = 670 +/- 10nm BP

(MoFlo XDP FL6 fluorochrome options/examples - APC, Alexa Fluor 647, Cy5)

 

FL7 = 740+nm LP

(MoFlo XDP FL7 fluorochrome options/examples - APC-Cy7, Alexa 750)

* for more information on multicolor analysis see User Guidelines