Department of Pathology, Section of Anatomic Pathology
Office Phone: (706) 721-2925
Office Address: BF-212/BAE-2576
Vice Chairman for Translation Research
Chief, Section of Molecular and Genetic Pathology
Associate Director Residency Program
Director, Georgia Esoteric & Molecular Laboratory at Augusta University
MBBS, 2001: Mahatma Gandhi Medical College, Aurangabad, India.
PhD, 2006; Mississippi State University, Starkville, MS.
Residency and Postdoctoral Fellowship
2008 - 2012, Resident, Pathology AP/CP, Augusta University.
2011 - 2012, Chief Resident, Augusta University.
2007 - 2008, Post-Doctoral Fellowship, Augusta University
01/14 - 12/14, Fellowship, Teaching Scholars Tract, EII, Augusta University
- As a Molecular and Genetic Pathologist, I am actively engaged in molecular and cytogenetic
evaluation of patient samples as a part of the multi- disciplinary clinical team treating
patients. It is my goal to render not only the most accurate diagnosis but also to
provide and develop the highest quality predictive and prognostic tests as a part
of personalized medicine. This approach helps my clinical colleagues take the best
possible care of the patient while at the same time advancing our scientific knowledge
in these areas. My clinical work involves molecular evaluation of various pathologic
disorders and malignancies.
- As a CLIA Laboratory Director, I am involved in identifying and validating newer cutting-edge
platforms for diagnostic medicine. I am actively collaborating with industry partners
and platform manufacturers to develop, validate new instruments for pathology. I am
also actively involved in medical and graduate education and translation pathology
- Surgical Pathology
- Breast Pathology
- Molecular Pathology and Cancer Cytogenetics
- Pathology Informatics
Certification and Licensures
- Diplomate of the American Board of Pathology: Anatomic and Clinical Pathology.
- Fellow College of American Pathology (FCAP).
- Fellow American Society of Clinical Pathology (FASCP).
- Georgia State Medical License (Georgia State Board of Medical Examiners): Active and
- Clinical Laboratory Director License (CLIA), Georgia Department of Community Health,
State of Georgia.
SARS2 SeroPrevalence And Respiratory Tract Assessment (SPARTA) (NIAIID Study )
The goal of this project is to understand the immune responses that emerge in response
to this Sars-COV-2 infection in frontline workers. We will measure when immune responses
develop, how long they last, and, importantly, if antibodies to SARS-CoV-2 or other
immune system components provide immune protection from re-infection. Further, we
will examine immunological, demographic, and medical risk factors for infection outcome
and examine immune responses to infection across the disease spectrum. As vaccines
become available for the population, we also want to analyze the antibody responses
specific to vaccine administrations.
Adaptive Pooling for faster COVID-19 testing
The goal of this project is to develop a pooling algorithm for COVID19 testing to
provide confirmed results of a greater number of samples in a single round of testing
(up to 200:1), increasing throughput and using fewer resources.
Understanding the role of JAK inhibition (with Ruxolitinib) on Donor T-cell population
GVHD is a major complication of allogeneic HSCT caused by alloreactive donor T cells.
After bone marrow transplantation, the alloreactive donor T cells recognize the alloantigens
presented by MHC in the recipients and initiate the pathogenesis of GVHD. The contribution
of different subsets of donor T cells to GVHD is different. The overarching goal of
this project is to investigate by pulsing donor cells with Ruxolitinib in the presence
or absence of G-CSF, donor cells do not cause GVHD or cause only minor GVHD after
Utility of IL-6 Measurement in Hospitalized COVID-19 Patients as a Screening Tool
for Cytokine-Release Syndrome and Multi-Organ Failure
The goal of this study is to evaluate the utility and role of the IL-6 kit to identify
severe inflammatory responses in patients with confirmed COVID-19 illness and to aid
in determining the risk of intubation with mechanical ventilation, in conjunction
with clinical findings and the results of another laboratory testing.
Cellular Heterogeneity and Tumor Microenvironment in Triple-Negative Breast Cancer
The goal of this project is to develop innovative therapeutic strategies targeting
tumor cells and the tumor microenvironment (TME) in triple-negative breast cancer
(TNBC), which disproportionally affects African American and other minority populations.
We hypothesize that differential cellular subpopulations comprising tumor cells and
the TME may contribute to disparate TNBC prevalence and treatment outcomes.
Devising and developing new molecular laboratory tests (LDT).
-Identifying newer validated platforms and incorporating them in the development of
advanced molecular testing in human diseases.
-Performing CLIA validation, applying for PLA codes for new technologies.
- Medical Students: Phase II pathology – Cytogenetics of myeloproliferative neoplasms, Breast pathology,
- Phase I Cellular and Molecular Basis of Medicine module- Personalized Medicine
- Pathology Residents: Molecular Pathology, Surgical Pathology, Cancer Cytogenetics, Breast Pathology, Lab
- Hematology-oncology Fellows: Molecular Pathology, Cancer Cytogenetics.
- Med Tech students - Clinical Molecular Methods Lab, Clinical Molecular Methods Internship CLSC 7990
- PRIDE Summer Institute: Molecular Diagnostics.
- Artificial Intelligence in Medicine: https://www.augusta.edu/mcg/medicine/ai/
- Pandemic Medicine Elective
- Pankaj Ahluwalia, Kumar Vaibhav, Meenakshi Ahluwalia, Ashis Kumar Mondal, Nikhil Sahajpal,
Amyn M. Rojiani and Kolhe, Ravindra. "Infection and immune memory: variables in robust protection by vaccines against
SARS-CoV-2." Frontiers in Immunology 12 (2021): 1756.
- Sahajpal, N. S. S., Mondal, A. K., Ananth, S., Njau, A., Ahluwalia, P., Newnam, G.,
... & Kolhe, R. (2020). SalivaAll: Clinical validation of a sensitive test for saliva collected in
healthcare and community settings with pooling utility for SARS-CoV-2 mass surveillance.
The Journal of Molecular Diagnostics JMDI-D-20-00497R3.
- Boyle, T. A., Mondal, A. K., Saeed-Vafa, D., Ananth, S., Ahluwalia, P., Kothapalli,
R., ... & Kolhe, R. (2021). Guideline adherent clinical validation of a comprehensive 170-gene DNA/RNA
panel for determination of small variants, copy number variations, splice variants,
and fusions on a next-generation sequencing platform in the CLIA setting. Frontiers in Genetics, 12 (2021): 725. doi: 10.3389/fgene.2021.503830.
- Sahajpal, Nikhil Shri, Hayk Barseghyan, Ravindra Kolhe, Alex Hastie, and Alka Chaubey. "Optical genome mapping as a next-generation cytogenomic
tool for detection of structural and copy number variations for prenatal genomic analyses."
Genes 12, no. 3 (2021): 398.
- Ahluwalia, Pankaj, Meenakshi Ahluwalia, Ashis K. Mondal, Nikhil Sahajpal, Vamsi Kota,
Mumtaz V. Rojiani, Amyn M. Rojiani, and Ravindra Kolhe. "Prognostic and therapeutic implications of an extracellular matrix-associated gene
signature in renal clear cell carcinoma." Scientific Reports 11, no. 1 (2021): 1-14.
- Kolhe, Ravindra, Virgenal Owens, Ashok Sharma, Tae Jin Lee, Wenbo Zhi, Umar Ghilzai, Ashis K. Mondal
et al. "Sex-Specific Differences in Extracellular Vesicle Protein Cargo in Synovial
Fluid of Patients with Osteoarthritis." Life 10, no. 12 (2020): 337.
- Ahluwalia, P., M. Ahluwalia, A. K. Mondal, N. Sahajpal, V. Kota, M. V. Rojiani, A.
M. Rojiani, and Kolhe. "Immunogenomic Gene Signature of Cell-Death Associated Genes with Prognostic Implications
in Lung Cancer. Cancers 2021, 13, 155." (2021).
- Ahluwalia, P., Ahluwalia, M., Vaibhav, K., Mondal, A., Sahajpal, N., Islam, S., ...
& Kolhe R. (2020). Infections of the lung: a predictive, preventive, and personalized perspective
through the lens of evolution, the emergence of SARS-CoV-2 and its pathogenesis. EPMA
Journal, 11(4), 581-601.
- Sahajpal, Nikhil Shri, Ashis K. Mondal, Allan Njau, Sudha Ananth, Salil Ghamande,
Madhuri Hegde, Alka Chaubey, Amyn M Rojiani, and Ravindra Kolhe. "COVID-19 screening in a healthcare or community setting: the complexity of saliva
as a specimen for PCR-based testing." Future Medicinal Chemistry (2020).
- Sahajpal, N. S., Mondal, A. K., Ananth, S., Njau, A., Ahluwalia, P., & Kolhe, R. (2020). Clinical performance and utility of a comprehensive next-generation sequencing
DNA panel for the simultaneous analysis of variants, TMB, and MSI for myeloid neoplasms.
PLoS ONE, 15(10), e0240976.
- Sahajpal, N.S., Mondal, A.K., Njau, A., Ananth, S., Jones, K., Ahluwalia, P.K., Ahluwalia,
M., Jilani, Y., Chaubey, A., Hegde, M. and Kolhe R., 2020. Proposal of RT-PCR–Based Mass Population Screening for Severe Acute Respiratory
Syndrome Coronavirus 2 (Coronavirus Disease 2019). The Journal of Molecular Diagnostics,
- S Sahajpal, N., Mondal, A.K., Njau, A., Ananth, S., Jones, K., Ahluwalia, P.K., Ahluwalia,
M., Jilani, Y., Chaubey, A., Hegde, M. and Kolhe R., Effective optimization of SARS-CoV-2 laboratory testing variables in an era of supply
chain constraints. Future microbiology, 15(15), pp.1483-1487.
- Sahajpal, Nikhil Shri, Allan Njau, Ashis K. Mondal, Sudha Ananth, Alka Chaubey, Amyn
Rojiani, and Ravindra Kolhe. "Role of clinical laboratories in response to the COVID-19 pandemic." Future Med
Chem. 2020;10.4155/fmc-2020-0129. doi:10.4155/fmc-2020-0129 (2020): 1521-1523.
- Kolhe R, Hunter M, Liu S, Jadeja RN, Pundkar C, Mondal AK, Mendhe B, Drewry M, Rojiani MV,
Liu Y, Isales CM. Gender-specific differential expression of exosomal miRNA in synovial
fluid of patients with osteoarthritis. Scientific Reports. 2017 May 17;7(1):2029.
For a complete listing of all publications, see Dr. Kolhe's work here.