The Mei Lab

Protocols

Biochemistry

  • Elisa assay
  • Immunoisolation of synaptic vesicles
  • Synaptosome preparation

Cell Biology

  • Brain cell culture Astroglial cell culture
    1. Astroglial cell culture
    2. Axon outgrowth assay by explant culture
    3. Cell culture N19 & N20
    4. Cerebellar granule neuron culture and migration recording
    5. Cortical neuron culture
    6. Glial cell cultures
    7. Hippocampal cell culture
    8. Oli-neu culture
    9. Xenopus neurite culture
  • Muscle cell culture
    • C2C12 cell culture
    • Chick embryo muscle culture
    • Primary rat muscle culture

Electron Microscope

  • EM protocol for mouse diaphragm

Histology

  • BrdU labeling
  • Immunochemistry for neuron culture
  • In Situ( E18 diaphragm mouse)
  • Whole mount staining (mouse diaphragm)

Molecular Biology

  • DNARNA
    • Cell transfection with calcium phosphate
    • Electrical stimulation of embryonic rat primary skeletal mus
    • Generation of recombinant adenoviruses
    • Luciferase assay
    • Retrovirus transient transfection of 293GPG packaging cells
    • RT-PCR
    • Subcloning
    • Transformation
  • Protein
    • Beta galactosidase assay
    • Biotinylation of cell surface proteins
    • CAT assay
    • GST- fusion protein purification and pull down assay
    • Immunoprecipitation
    • PAK1 kinase assay
    • PLC assay
    • Recepter endocytosis
    • RHO GAP assay
    • Western blotting
    • Yeast two-hybrid