Core Faculty


Tohru Fukai, PhD.

Masuko Ushio-Fukai, Ph.D., FAHA

Professor
Department of Medicine, Cardiology
Director of the Redox Signaling Program

More about Dr. Ushio-Fukai


Contact Info

Phone: (706) 721-9728
Fax: (706) 721-9799
Email: mfukai@augusta.edu
Office: CB 3212A
Lab: CB-3201B


Education and Training

Postdoctoral Training
Emory University
Atlanta , GA.

Ph.D.
Kyushu University
Fukuoka, Japan

M.S.
Kyushu University
Fukuoka, Japan

B.S.
Kyushu University
Fukuoka, Japan


Society Memberships

  • 2012
    Member, American Physiology Society
  • 2007
    International Society for Stem Cell Research
  • 2005
    Council, High Blood Pressure Research, American Heart Association
  • 2001
    Council, Arteriosclerosis, Thrombosis, and Vascular Biology, American Heart Association
  • 2001-present
    Council for High Blood Pressure Research, American Heart Association,
  • 1999
    NAVBO: North American Vascular Biology Organization,
  • 1999
    Council, Basic Cardiovascular Science, American Heart Association,
  • 1999
    The International Society for Free Radical Research
  • 1988
    American Association for the Advancement of Science (AAAS)


Research

My research is focused on the molecular mechanism of angiogenesis and vasculogenesis, which play an important role for development, wound repair and regenerative medicine required for treatment of ischemic heart/limb disease. Uncontrolled angiogenesis also contributes to atherosclerosis, inflammatory disease and cancer. During the past 17 years, my laboratory has devoted efforts to address the role of reactive oxygen species (ROS) in mediating signal transduction, so called “redox signaling” in vascular biology and cardiovascular/metabolic disease. My laboratory is one of the first to demonstrate that NADPH oxidase (NOX) and endothelial cell (EC)-derived H2O2 play an essential role in post-ischemic neovascularization. Using yeast two-hybrid system, we identified IQGAP1, as a novel VEGFR2-binding scaffold protein, involved in compartmentalized ROS signaling at leading edge driving directional EC migration (invited review in Science STKE 2006), which promotes post-ischemic neovascularization.  To understand how diffusible ROS derived from NOX and mitochondria activate specific signaling pathways and to identify the molecular targets of ROS involved in angiogenesis, we apply innovative redox proteomics approach using the newly-developed Cys-OH trapping reagents during angiogenesis in vitro and in vivo. Furthermore, we investigates the redox regulation of stem/progenitor cell function as well as stem cell niche in bone marrow. Moreover, we also study a role of copper (Cu) transport proteins, which have redox-sensitive Cu binding CXXC motifs, in ROS-dependent angiogenesis and tissue repair in obese/diabetes models. My lab uses molecular cell biology and biochemical approaches, cell imaging and signal transduction analysis and various genetic mice models. By understanding the redox signaling and biology, we hope to develop new therapies for treating manifested cardiovascular and metabolic disease as well as cancer.



Projects

Redox Signaling and Endothelial Cell Metabolism in Angiogenesis.

Mitochondria Dynamics and Autophagy in Impaired Angiogenesis in Metabolic Diseases.

Copper Transport Proteins in Angiogenesis and Diabetic Vascular Complications.

Redox Regulation of Stem/Progenitor Cell Function and Bone Marrow Stem Cell Niche.

Mechanism of Exercise on improving Endothelial Function and Angiogenesis



Lab

Sudhahar Varadarajan

Sudhahar Varadarajan, PhD
Assistant Professor
Role of Cu transport proteins and reactive oxygen species in atherosclerosis, aortic aneurysm, hypertension, and diabetes.

Archita Das

Archita Das, PhD
Postdoctoral Fellow
Role of Cu transport proteins and reactive oxygen species in atherosclerosis, aortic aneurysm, hypertension, and diabetes.

Dipankar Ash

Dipankar Ash, PhD
Postdoctoral Fellow
Role of Copper Transport protein in Angiogenesis and Autophagy.

Kareem Abdelsaid

Kareem Abdelsaid

Yali Hou

 Yali Hou

Xuexiu Fang

 Xuexiu Fang

Maggie McMenamin

 Maggie McMenamin

Sheela Nagarkoti

 Sheela Nagarkoti



Publications

Go to PUBMED for more publications

Kim YM, Kim SJ, Tatsunami R, Yamamura H, Fukai T, Ushio-Fukai M. ROS-induced ROS Release Orchestrated by Nox4, Nox2 and Mitochondria in VEGF Signaling and Angiogenesis.

Am J Physiol Cell Physiol. 2017 Apr 19 (Epub ahead of print)

Das A, Sudhahar V, Chen GF, Kim HW, Youn SW, Finney L, Vogt S, Yang J, Kweon J, Surenkhuu B, Ushio-Fukai M, Fukai T. Endothelial Antioxidant-1: a Key Mediator of Copper-dependent Wound Healing in vivo. Sci Rep. 2016; 6:33783.

Delaney MK, Kim K, Estevez B, Xu Z, Stojanovic-Terpo A, Shen B, Ushio-Fukai M, Cho J, Du X. Differential Roles of the NADPH-Oxidase 1 and 2 in Platelet Activation and Thrombosis. Arterioscler Thromb Vasc Biol. 2016;36:846-54

Chen GF, Sudhahar V, Youn SW, Das A, Cho J, Kamiya T, Urao N, McKinney RD, Surenkhuu B, Hamakubo T, Iwanari H, Li S, Christman JW, Shantikumar S, Angelini GD, Emanueli C, Ushio-Fukai M*, Fukai T*. Copper Transport Protein Antioxidant-1 Promotes Inflammatory Neovascularization via Chaperone and Transcription Factor Function. Sci Rep. 2015;5:14780. (*equally contributed corresponding author)

Mittal M, Urao N, Hecquet CM, Zhang M, Sudhahar V, Gao XP, Komarova Y, Ushio-Fukai M, Malik AB. Novel role of reactive oxygen species-activated Trp melastatin channel-2 in mediating angiogenesis and postischemic neovascularization. Arterioscler Thromb Vasc Biol. 2015; 35: 877-87.

Ushio-Fukai M, Rehman J. Redox and metabolic regulation of stem/progenitor cells and their niche. Antioxid Redox Signal. 2014;21:1587-90.

Yamada KH, Nakajima Y, Geyer M, Wary KK, Ushio-Fukai M, Komarova Y, Malik AB. KIF13B regulates angiogenesis through Golgi to plasma membrane trafficking of VEGFR2. J Cell Sci. 2014;127:4518-30.

Urao N, Sudhahar V, Kim SJ, Chen GF, McKinney RD, Koida G, Fukai T, Ushio-Fukai M. Critical role of endothelial hydrogen peroxide in post-ischemic neovascularization. PLoS One. 8:e57618, 2013.

Urao N, Ushio-Fukai M. Redox regulation of stem/progenitor cells and bone marrow niche. Free Radic Biol Med. 54:26-39, 2013.

Spotlight Publications 

Ushio-Fukai M. Localizing NADPH oxidase-derived ROS. Sci STKE. 2006 (349):re8. (Invited review)

 Griendling KK, Sorescu D, Ushio-Fukai M. NAD(P)H Oxidase: role in cardiovascular biology and disease. Circ Res. 2000: 86: 494-501. Cited by 3056.