The Georgia Cancer Center Immune Monitoring Shared Resource (IMSR) was established
The mission of the IMSR laboratory is to provide comprehensive immune monitoring services
and expertise to assist researchers and clinicians in designing and performing basic
and translational research projects. The main objective of the core is to provide
standardized immune-based assays, to develop, optimize, and validate new technologies,
and to support efforts in the areas of human and animal immunology and immunotherapy.
The IMSR works closely with the Flow Cytometry Core, the Integrated Genomic Shared
Resources, and the Tumor Tissue and Serum Repository at the Georgia Cancer Center.
Currently the IMSR offers:
- Immune cell phenotyping by flow cytometry, including intracellular cytokine staining
- Human TCR repertoire sequencing
- Immune cell isolation
- Soluble protein detection by Cytometric Bead Array (CBA)
- Technical training, data analysis, and assistance for experimental design, collaborative
clinical project development, and grant/manuscript preparation.
The core is also developing Luminex, ELISA, and cellular functional assays such as
a Treg suppression assay.
Please see our iLab website for currently available services and detail information including fees.
Experimental design discussion
Teach techniques of sample preparation, FACS staining procedures, use of software
for data analysis
- Data Analysis
Custom data processing and analysis
- Grant/Manuscript Assistance
Preparation of manuscript or grant applications using service provided by the IMSR
Flow-based Bead Array
- Measurement of soluble proteins (up to 13 per sample) in supernatants using flow-based
bead array technology (CBA from BD Biosciences or LEGENDplex from BiolLegend)
Immune Cell Phenotyping by Flow cytometry
- Multi-color flow cytometric analysis allows characterization of the immune phenotypes
(e.g. immune cell subsets, activation & differentiation status) of different immune
cell populations in human and animal models. We offer custom designed panels based
on each customer needs.
- Combination of tetramer/pentamer staining allows monitoring of antigen-specific CD8
- Intracellular staining of transcription factors (e.g. Foxp3) and cytokines allows
to determine simultaneously the phenotype of cells and their cytokine production profile.
We also offer several options for in vitro stimulation (e.g. LPS, PMA+Ionomycin, anti-CD3
and CD28 stimulation) of PBMCs for cytokine profiling.
Immune Cell Isolation
- PBMC isolation from whole blood samples
- Magnetic-beads based isolation of immune cell subsets (e.g. T cells, B cells and monocytes)
- For non-human cell isolation, please inquire.
NexGen TCR Sequencing
- Currently we offer high throughput sequencing of human T cell receptor (TCR) from
RNA or DNA.
- From RNA - simultaneus preparation of barcoded TCRalfa and TCRbeta library (PCR-unbiased,
using template-switching RT PCR technology) for Illumina Mi-SEQ PE sequencing
- From genomic DNA - preparation of barcoded TCRbeta targeted library for Illumina Mi-SEQ
- Service includes libraries pooling, MI-SEQ sequencing in collaboration with GCC Integrated
Genomics Core, QC, quantitation, CDR3 clones extraction and annotation (alignment,
error correction, V(D)J and CDR3 assignment). We also offer high level TCR analysis
including diversity measure, frequency distribution, clone tracking etc.
- For mouse TCR repertoire, please inquire.