The Pyrosequencer is used to detect and quantify genetic variations within short, targeted DNA sequences. It can detect low-level variations in multiple samples quickly, making it cost effective and efficient. The biotinylated single-stranded DNA template is loading on a plate, and the machine adds dNTPs. The dNTPs release pyrophosphate when attached to the template, allowing for a signal to be given off in respect to which dNTP and the quantity that is incorporated.
* $10 per run, if providing your samples biotinylated. The instrument can run up to 96 samples in one setting, and allows for easy labeling.
Sample preparation must follow Qiagen’s protocols.