The Biochemistry Laboratory contains state-of-the-art equipment for biochemical analyses. These include chambers
for histopathology, an operating hood for animal surgery, gel electrophoresis devices,
fume hoods, -800C deep freezers, dry ice facilities, 40C refrigerator, two centrifuges,
high-speed centrifuge, Mettler balance, ultra centrifuges, student microscope, photomicroscope,
inverted microscope with a fluorescence attachment, gel scanner, plate reader (spectrophotometer
and fluorometer), operating microscopes, stacking incubator, tissue culture hoods,
and a Millipore water filtration apparatus. Shared equipment include a photographic
darkroom for developing autoradiograms of the Western blots, cold and warm rooms,
wet ice and autoclave facilities.
The focus of my research include trauma to the nervous system and neural repair. My current collaborations on the Augusta University campus, spanning several colleges and departments, have allowed me to develop novel areas of clinical research. I have been engaged in the field of CNS injury and its amelioration for number of years. I have worked on various animal stroke models including MCAo, SAH and TBI models in rats and mice. My recent work focuses on studying role of inflammation in CNS injury and neurodegenerative diseases in patients.
• Cytokine induced inflammatory cascade in neural injury and repair
• The role of GPR109a receptors in ameliorating hypoxic brain injury
Giri, B., Belanger, K., Bradley, E., Chong, R.K., Morgan, J., Baban, B., & Wakade, C. Niacin abrogates inflammatory cytokine upregulation through G-protein coupled receptor GPR109A. Submitted.
Chong, R.K., Albor, L., Wakade, C., & Morgan, C. The dimensionality of fatigue in Parkinson's disease. Submitted.
Baban, B., Giri, B., Malik, A., Morgan, J., Chong, R.K. & Wakade, C. (2018). Niacin modulates macrophage polarization in Parkinson's disease. Journal of Neuroimmunology, 320, 76-79. doi: 10.1016/j.jneuroim.2018.05.002
Baban, B., Giri, B., Malik, A., Morgan, J., Chong, R.K. & Wakade, C. (2018). Niacin modulates macrophage polarization in Parkinson's disease. Journal of Immunology. In press.
Wakade, C., *Chong, R.K., Bradley, E. & Morgan, J. (2015). Low-dose niacin supplementation modulates GPR109A levels and NAD/NADH ratio and ameliorates PD symptoms. Clinical Case Reports. doi: 10.1002/ccr3.232 *Co-first & corresponding author
Chong, R.K., Lee, K.H., Morgan, J., & Wakade, C. (2015). Duration of step initiation
predicts freezing in Parkinson's disease. Acta Neurologia Scandinavica, DOI: 10.1111/ane.12361.
Wakade, C., & *Chong, R.K. (2014). A novel treatment target for Parkinson’s disease. Journal of the Neurological Sciences, 347, 34-38. http://dx.doi.org/10.1016/j.jns.2014.10.024 *Co-first & corresponding author
Wakade, C., *Chong, R.K., Bradley, E., Thomas, B., Morgan, J. (2014). Upregulation
of GPR109A in Parkinson’s disease. PLoS ONE, 9(10), e109818. http://dx.doi.org/10.1371/journal.pone.0109818 *Co-first & corresponding author
Chong, R.K., Adams, K., Fenton, K., Gibson, M., Hodges, K., Horne, J., & Wakade, C. (2014). Postural adaptation to a slow sensorimotor set-changing task in Parkinson's disease. Comprehensive Psychology, 3(1), Article 9. http://dx.doi.org/10.2466/15.26.CP.3.9
Wakade CG, King MD, Laird MD, Alleyne CH Jr, Dhandapani KM. Curcumin attenuates vascular
inflammation and cerebral vasospasm after subarachnoid hemorrhage in mice. Antioxid
Redox Signal. 2009;11(1):35-45.
Laird MD, Wakade CG, Alleyne CH Jr, Dhandapani KM. Hemin-induced necroptosis involves glutathione depletion in mouse astrocytes. Free Radic Biol Med. Oct 15;45(8):1103-14, 2008. Epub 2008 Jul 16.
Wakade CG, Khan MM, De Sevilla LM, Zhang QG, Mahesh VB, Brann DW. Tamoxifen neuroprotection in cerebral ischemia involves attenuation of kinase activation and superoxide production and potentiation of mitochondrial superoxide dismutase. Endocrinology. Jan;149(1):367-79, 2008 Epub 2007 Sep 27.
Dhandapani KM, Khan MM, Wade FM, Wakade CG, Mahesh VB and Brann DW. Induction of transforming growth factor-B1 by basic fibroblast growth factor in glioma and astrocytes is mediated by MEK/ERK signaling and AP-1 activation. Neurosci Res. Apr;85(5):1033-45, 2007.
Brann DW, Dhandapani KM, Wakade CG, Mahesh VB, Khan MM. Neurotrophic and neuroprotective actions of estrogen: basic mechanisms and clinical implications. Steroids. May 72(5);381-405. Epub 2007 Feb 21, 2007.
Khan MM, Hadman M, Wakade CG, De Sevilla LM, Dhandapani KM, Mahesh VB, Vadlamudi RK, Brann DW. Cloning of rat MNAR/PELP1: Localization in rat brain and colocalization in estrogen receptor-? but not GnRH-positive neurons. Endocrinology, Dec:146(12):5215-27. Epub 2005.
Dhandapani KM, Wade FM, Wakade CG, Mahesh VB, Brann DW. Neuroprotection by stem cell factor in rat cortical neurons involves Akt and NF?B. Journal of Neurochemistry. 2005;95(1):9-19.
Irons H, Lind JG, Wakade CG, Yu G, Hadman M, Carroll J, Hess DC and Borlongan CV. Intracerebral xenotransplantation of gfp mouse bone marrow stromal cells in intact and stroke rat brain: graft survival and immunologic response. Cell Transplantation. 3(3):283-94, 2004.
Intracerebral xenotransplantation of GFP mouse bone marrow stromal cells in intact and stroke rat brain: graft survival and immunologic response. Irons H, Lind JG, Wakade CG, Yu G, Hadman M, Carroll J, Hess DC, Borlongan CV. Cell Transplant. 2004;13(3):283-94.
Atypical neuroleptics stimulate neurogenesis in adult rat brain. Wakade CG, Mahadik SP, Waller JL, Chiu FC. J Neurosci Res. 2002;69(1):72-9.
The NF-kappaB inhibitor diethyldithiocarbamate (DDTC) increases brain cell death in a transient middle cerebral artery occlusion model of ischemia. Hill WD, Hess DC, Carroll JE, Wakade CG, Howard EF, Chen Q, Cheng C, Martin-Studdard A, Waller JL, Beswick RA. Brain Res Bull. 2001;55(3):375-86.
Nuclear factor-kappa B activation during cerebral reperfusion: effect of attenuation with N-acetylcysteine treatment. Carroll JE, Howard EF, Hess DC, Wakade CG, Chen Q, Cheng C. Brain Res Mol Brain Res. 1998;56(1-2):186-91.
Plasma membrane phospholipid and cholesterol distribution of skin fibroblasts from drug-naive patients at the onset of psychosis. Mahadik SP, Mukherjee S, Correnti EE, Kelkar HS, Wakade CG, Costa RM, Scheffer R. Schizophr Res. 1994;13(3):239-47.
Decreased adhesiveness and altered cellular distribution of fibronectin in fibroblasts from schizophrenic patients. Mahadik SP, Mukherjee S, Wakade CG, Laev H, Reddy RR, Schnur DB. Psychiatry Res. 1994;53(1):87-97.
Temporal changes in superoxide dismutase, glutathione peroxidase, and catalase levels in primary and peri-ischemic tissue. Monosialoganglioside (GM1) treatment effects. Mahadik SP, Makar TK, Murthy JN, Ortiz A, Wakade CG, Karpiak SE. Mol Chem Neuropathol. 1993;18(1-2):1-14.
Temporal changes in edema, Na+, K+, and Ca++ in focal cortical stroke: GM1 ganglioside reduces ischemic injury. Karpiak SE, Wakade CG, Tagliavia A, Mahadik SP. J Neurosci Res. 1991;30(3):512-20.
GM1 ganglioside treatment reduces functional deficits associated with cortical focal ischemia. Bharucha VA, Wakade CG, Mahadik SP, Karpiak SE. Exp Neurol. 1991;114(1):136-9.
GM1 ganglioside reduces cognitive dysfunction after focal cortical ischemia. Ortiz A, MacDonall JS, Wakade CG, Karpiak SE. Pharmacol Biochem Behav. 1990;37(4):679-84.
Ganglioside reduction of ischemic injury. Karpiak SE, Mahadik SP, Wakade CG. Crit Rev Neurobiol. 1990;5(3):221-37.
Animal models for the study of drugs in ischemic stroke. Karpiak SE, Tagliavia A, Wakade CG. Annu Rev Pharmacol Toxicol. 1989;29:403-14.
McN-A-343, a specific agonist of M1-muscarinic receptors, exerts antinicotinic and antimuscarinic effects in the rat adrenal medulla. Wakade AR, Kahn R, Malhotra RK, Wakade CG, Wakade TD. Life Sci. 1986;39(22):2073-80.
■ ■ ■ ■ ■